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Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications

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Methods in Membrane Lipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1232))

Abstract

A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of the event.

In this protocol we discuss the application of fluorescence recovery after photobleaching (FRAP) to study the dynamics of membrane associated molecules. We discuss the principles, acquisition and the analysis of FRAP data and address issues surrounding its interpretation.

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Acknowledgements

The authors would like to thank the Mark Wainwright Analytical Centre, University of New South Wales for continuing support.

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Authors and Affiliations

  1. Biomedical Imaging Facility, Lowy Cancer Research Centre, University of New South Wales, High Street, Kensington, Sydney, NSW, 2052, Australia

    Michael Carnell, Alex Macmillan & Renee Whan

Authors
  1. Michael Carnell
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  2. Alex Macmillan
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  3. Renee Whan
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Corresponding author

Correspondence to Renee Whan .

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Editors and Affiliations

  1. Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom

    Dylan M. Owen

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Carnell, M., Macmillan, A., Whan, R. (2015). Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications. In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-1752-5_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1751-8

  • Online ISBN: 978-1-4939-1752-5

  • eBook Packages: Springer Protocols

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