Summary
Following alloxan induced diabetes in rats, transplantation of neonatal pancreases under the kidney capsule was successfully carried out. The insulin response to oral ingestion of 150 and 750 mg of glucose was studied. The responses in controls and in rats 40 days after transplantation demonstrated a load-dependent increment of plasma insulin responses which was not related to the similar glucose responses. In control rats a part of the insulin response had occurred at 1 min, i. e. 2 min before the rise in blood glucose. After transplantation in the absence of this nervously triggered response, blood glucose rose faster than in control rats from 3 to 5 min after start of ingestion (p<0.01). In non-transplanted rats regenerating a part of their original pancreas within one week, glucose intolerance was seen 10 min after glucose ingestion, probably due to the lack of adequate secondary phase release. This study shows that maintenance of a normal glucose tolerance curve to glucose ingestion depends on at least two factors. First, an anticipatory nervously triggered insulin secretion. Second, a load dependent humoral potentiation of glucose stimulated insulin release.
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Strubbe JH, Bouman PR (1978) Plasma insulin patterns in the unanesthetized rat during intracardial infusion and spontaneous ingestion of graded loads of glucose. Metabolism 27:341–351
Cerasi E, Luft R, Efendic S (1972) Decreased sensitivity of the pancreatic beta cell to glucose in prediabetic and diabetic subjects. A glucose dose-response study. Diabetes 21:224–234
Efendic S, Cerasi E, Luft R (1974) Quantitative study on the potentiating effect of arginine on glucose-induced insulin response in healthy, prediabetic and diabetic subjects. Diabetes 23:161–171
Cerasi E, Efendic S, Luft R (1973) Dose-response relation between plasma-insulin and blood glucose levels during oral glucose loads in prediabetic and diabetic subjects. Lancet 1:794–797
Strubbe JH, Steffens AB (1975) Rapid insulin release after ingestion of a meal in the unanesthetized rat. Am J Physiol 229:1019–1022
De Jong A, Strubbe JH, Steffens AB (1977) Hypothalamic influence on insulin and glucagon release in the rat. Am J Physiol 223:380–388
Fischer U, Hommel H, Freyse EJ, Fiedler H (1975) Der Mechanismus der Insulinsekretion nach oraler Glukoseverabfolgung. IV. Verhinderung der reflektorischen Frühphase des Plasmainsulin-Anstieges durch Atropin. Endokrinologie 65:91–102
Fischer U, Hommel H, Ziegler M, Nowak W, Hahn von Dorsche H (1976) The mechanism of insulin secretion after oral glucose administration. VIII. Pancreatic juice insulin excretion after glucose loading and meal ingestion in normal and vagotomized dogs. Endokrinologie 68:327–337
Fischer U, Hommel H, Nowak W, Hahn von Dorsche H, Sill U, Lippert H (1976) Der Einfluß der Vagotomie auf die Glukosetoleranz sowie die Reaktionen von Plasmainsulin und exokriner Pankreasfunktion nach Glukosebelastung oder Nahrungsaufnahme bei Hunden. Acta Biol Med Germ 35:1279–1291
Strubbe JH, van Wachem P (1981) Insulin secretion by the transplanted neonatal pancreas during food intake in fasted and fed rats. Diabetologia 20:228–236
Louis-Sylvestre J (1978) Feeding and metabolic patterns in rats with truncular vagotomy or with transplanted B cells. Am J Physiol 235:119–125
Trimble ER, Siegel EG, Berthoud HR, Renold AE (1980) Intraportal islet transplantation: Functional assessment in conscious unrestrained rats. Endocrinology 106:791–797
Brown J, Clark WR, Molnar IG, Mullen YS (1976) Fetal pancreas transplantation for reversal of streptozotocin-induced diabetes in rats. Diabetes 25:56–64
Steffens AB (1969) A method for frequent sampling of blood and continuous infusion of fluids in the rat without disturbing the animal. Physiol Behav 4:833–836
Hegre OD, Leonard RJ, Erlandson SL, McEvoy RC, Parsons JA, Elde RP, Lazarow A (1976) Transplantation of islet tissue in the rat. Acta Endocrinol (Suppl) (Copenh) 83:257–281
Chisholm GJ, Young JD, Lazarus L (1969) The gastrointestinal stimulus to insulin release. I. secretin. J Clin Invest 48:1453–1459
Pederson RA, Brown JC (1979) Effect of cholecystokinin secretin and gastric inhibitory polypeptide on insulin release from the isolated perfused rat pancreas. Can J Physiol Pharmacol 57:1233–1237
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Strubbe, J.H. Effects of pancreas transplantation on insulin secretion in the rat during ingestion of varying glucose loads. Diabetologia 22, 354–357 (1982). https://doi.org/10.1007/BF00253581
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DOI: https://doi.org/10.1007/BF00253581