Summary
CrCl3, 0.25, 0.5, 1.0, and 1.5 mmol/l inhibited glucose-induced insulin secretion in a reversible and dose dependent manner. Cr also inhibited basal secretion of insulin in the presence of 5.5 mmol/l glucose and insulin secretion stimulated by 50 mmol/l K+ or 15 mmol/l L-leucine. When 2 mmol/l theophylline was employed to potentiate the stimulatory effect of 16.5 mmol/l glucose, the inhibitory effect of 1.5 mmol/l Cr was reduced and that of 0.5 mmol/l Cr virtually abolished. A similar reduction in the inhibitory effect of Cr was observed when the medium calcium concentration was increased from 2.5 to 5, 7.5 and 12.5 mmol/l. Cr did not alter the conversion of 14C-glucose to 14CO2 or 45Ca uptake by isolated islets. It is concluded that the inhibitory effect of Cr on insulin secretion may be mediated through interference with an intracellular function of Ca++ in the beta cell.
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References
Mertz W (1969) Chromium occurrence and function in biological systems. Physiol Rev 49: 163–239
Schroeder H A (1966) Chromium deficiency in rats: A syndrome simulating diabetes mellitus with retarded growth. J Nutr 88: 439–445
Levine R A, Streeten D J P, Doisy R J (1968) Effects of oral chromium supplementation on the glucose tolerance of elderly human subjects. Metabolism 17: 114–125
Sherman L, Glennon J A, Brech W J, Klomberg G H, Gordon E S (1968) Failure of trivalent chromium to improve hyperglycemia in diabetes mellitus. Metabolism 17: 439–442
Wise A (1978) Chromium supplementation and diabetes. JAMA 240: 2045–2046
Vakhrusheva V A (1960) Effect of manganese and chromium as trace elements on the level of blood sugar in white mice. Tr Izhevskogo Otd Vses Fisiol Obsh 1: 184–190
Ghafghazi T, Maghbareh A, Barnett R (in press) Chromium-induced hyperglycemia in the rat. Toxicology
Lacy P E, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35–39
Wright P H, Makulu D R, Vichick D, Sussman K E (1971) Insulin immunoassay by back-titration; some characteristics of the technic and the insulin precipitant action of alcohol. Diabetes 20: 33–45
Naber S P, McDaniel M L, Lacy P E (1977) The effect of glucose on the acute uptake and efflux of calcium-45 in isolated rat islets. Endocrinology 101: 686–693
McDaniel M L, King S, Anderson S, Fink J, Lacy P E (1974) Effect of cytochalasin B on hexose transport and glucose metabolism in pancreatic islets. Diabetologia 10: 303–308
Brisson G R, Malaisse-Lagae F, Malaisse W J (1972) The stimulus-secretion coupling of glucose-induced insulin release. VII. A proposed site of action for adenosine-3′,-5′-cyclic monophosphate. J Clin Invest 51: 232–241
Watkins D T, Moore M (1974) Effect of sulfhydrylbinding reagents on insulin release from isolated secretion granules. Endocrinology 95: 485–491
Ghafghazi T, Maghbareh A, Barnett R (1978) Effect of chromium on glucose tolerance in the rat. I R C S Med Sci 6: 396
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Ghafghazi, T., McDaniel, M.L. & Lacy, P.E. Chromium-induced inhibition of insulin secretion from isolated Islets of Langerhans. Diabetologia 18, 229–232 (1980). https://doi.org/10.1007/BF00251921
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DOI: https://doi.org/10.1007/BF00251921