Abstract
The radiotoxic effects of L-[methyl11C] methionine were estimated by measuring the accumulation of DNA strand breaks.CHO, Chinese-hamster cells, were incubated in 11C-methionine-containing medium for 60 min at 37°C. The number of unrepaired DNA strand breaks was then examined by the DNA-unwinding method. For comparison, cells were also externally irradiated under similar conditions with gamma radiation (137Cs) or positrons (11C). The relative biological effectiveness of 11C-methionine was estimated to be about 1.
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References
Apelgot S (1983) Some considerations of the transmutation effect due to radioactive isotopes incorporated in cells and on the irradiation effect. Int J Radiat Biol 43:95–101
Berger MJ (1971) Distribution of absorbed dose around point sources of electrons and beta particles in water and other media. MIRD pamphlet no 7. J Nucl Med (suppl) 5:5–23
Blöcher D, Pohlit W (1982) DNA double strand breaks in Ehrlich ascites tumour cells at low doses of X-rays. II. Can cell death be attributed to double strand breaks? Int J Radiat Biol 42:329–338
Brandt S (1976) Statistical and computational methods in data analysis. North Holland, Amsterdam Oxford New York, pp 33–38
Bryant PE (1984) Effects of araA and fresh medium on chromosome damage and DNA double-strand break repair in X-irradiated stationary cells. Br J Cancer 49:61–65
Bryant PE, Blöcher D (1980) Mesurement of the kinetics of DNA double strand break repair in Ehrlich ascites tumour cells using the unwinding method. Int J Radiat Biol 38:335–347
Burki HJ, Roots R, Feinendegen LE, Bond VP (1973) Inactivation of mammalian cells after disintegration of 3H or 125I in cell DNA at-196° C. Int J Radiat Biol 24:363–375
Chan PC, Lisco E, Lisco H, Adelstein SJ (1976) The radiotoxicity of iodine-125 in mammalian cells. II. A comparative study on cell survival and cytogenetic responses to 125IUdR, 131IUdR and 3H-TdR. Radiat Res 67:322–343
d'Argy R, Ullberg S, Stålnacke C-G, Långström B (1984) Wholebody autoradiography using 11C with double tracer applications. Int J Appl Radiat Isot 35:129–134
Dewey WC, Furman S, Miller HH (1970) Comparison of lethality and chromosomal damage induced by X-rays in synchronized Chinese hamster cells in vitro. Radiat Res 43:561–581
Dillman LT (1970) Radionuclide decay schemes and nuclear parameters for rise in radiation dose estimation. MIRD pamphlet No 6. J Nucl Med (suppl) 6:5–32
Ell PJ, Holman BL (1982) Computed emission tomography. Oxford University Press, Oxford
Fitzgerald JJ, Brownell GL, Mahoney FJ (1967) Mathematical theory of radiation dosimetry, Gordon and Breach, New York
Grote SJ, Revell SH (1972) Correlation of chromosome damage and colony forming ability in Syrian hamster cells in culture irradiated in G1. Curr Top Radiat Res 7:303–315
Hutchinson F (1965) The molecular basis for radiation effects on cells. Cancer Res 26:2045–2052
ICRU (1979) Methods of assessment of absorbed dose in clinical use of radionuclides. ICRU report no 32. ICRU Publications, Washington
Kassis AI, Adelstein SJ, Haydock C, Sastry KSR (1983) Thallium-201: An experimental and a theoretical radiobiological approach to dosimetry. J Nucl Med 24:1164–1175
Långström B, Lundqvist H (1976) The preparation of 11C-methyliodide and its use in the synthesis of (11C-methyl)-l-methionine. Int J Appl Radiat Isot 27:357–363
Lilja A, Bergström K, Hartvig P, Spännare B, Halldin C, Lundqvist H, Långström B (1985) Dynamic study of supratentorial gliomas with l-methyl-11C/methionine and positron emission tomography (PET). AJNR (in press)
Modig HG, Edgren M, Revesz L (1977) Effect of radioprotective aminothiols on the induction and repair of single-strand breaks in the DNA of irradiated mammalian cells. Acta Radiol [Oncol] 16:245–256
Obe G, Natarajan AT, Palitti F (1982) Role of DNA double strand breaks in the formation of radiation-induced chromosomal aberrations. In: Natarajan AT Obe G, Altman H (eds) Progress in mutation research, vol 4. Elsevier, Amsterdam, pp 1–9
Painter RB (1980) The role of DNA damage in repair in cell killing inducing by ionizing radiation. In: Meyn RE, Withers HR (eds) Radiation biology in cancer research. Raven Press, New York, pp 59–68
Pohlit W (1983) Lethal, sublethal and potentially lethal radiation damage. In: Broerse JJ, Barendsen GW, Kal HB, van der Kogel AJ (eds) Proceedings of the Seventh Congress of Radiation Research. Martinus Nijhoff, Amsterdam, pp 263–269
Roots R, Yang C, Craise L, Blakely EA, Tobias CA (1979) Impaired repair capacity of DNA breaks induced in mammalian cellular DNA by accelerated heavy ions. Radiat Res 78:38–49
Rydberg B (1975) The rate of strand separation in alkali of DNA of irradiated mammalian cells. Radiat Res 61:274–287
Stålnacke C-G, Jones B, Långström B, Lundqvist H, Malmborg P, Sjöberg S, Larsson B (1982) Short-lived radionuclides in nutritional physiology. A model study with l-(me-11C)methionine in the pig. Br J Nutr 47:537–545
Sundell-Bergman S, Johanson KJ (1980) Repairable and unrepairable DNA strand breaks induced by decay of 3H and 125I incorporated into DNA of mammalian cells. Radiat Environ Biophys 18:239–248
Wolff S (1972) Genetic effects and radiation induced cell death. Front Radiat Ther Oncol 46:459–469
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Stålnacke, CG., Sundell-Bergman, S., Halldin, C. et al. Radiotoxicity of 11C-methionine measured by the accumulation of DNA strand breaks in mammalian cells. Eur J Nucl Med 11, 166–170 (1985). https://doi.org/10.1007/BF00251368
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DOI: https://doi.org/10.1007/BF00251368