Abstract
Polyclonal antiserum specific for ferredoxin-nitrite reductase (EC 1.7.7.1) from the green alga Chlamydomonas reinhardii recognized the nitrite reductase from other green algae, but did not cross-react with the corresponding enzyme from different cyanobacteria or higher plant leaves. An analogous situation was also found for ferredoxin-glutamate synthase (EC 1.4.7.1), using its specific antiserum. Besides, the antibodies raised against C. reinhardii ferredoxin-glutamate synthase were able to inactivate the ferredoxin-dependent activity of nitrite reductase from green algae.
These results suggest that there exist similar domains in ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from green algae. In addition, both types of enzymes share common antigenic determinants, probably located at the ferredoxin-binding domain. In spite of their physicochemical resemblances, no apparent antigenic correlation exists between the corresponding enzymes from green algae and those from higher plant leaves or cyanobacteria.
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Abbreviations
- Fd:
-
ferredoxin
- GOGAT:
-
glutamate synthase
- MV+ :
-
reduced methyl viologen (radical cation)
- NiR:
-
nitrite reductase
- PMSF:
-
phenylmethylsulphonyl fluoride
- SDS:
-
sodium dodecyl sulfate
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Gotor, C., Pajuelo, E., Romero, L.C. et al. Immunological studies of ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from photosynthetic organisms. Arch. Microbiol. 153, 230–234 (1990). https://doi.org/10.1007/BF00249073
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DOI: https://doi.org/10.1007/BF00249073