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A pathway of the autotrophic CO2 fixation in Chloroflexus aurantiacus

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Abstract

Autotrophically grown cells of Chloroflexus aurantiacus B-3 were shown to possess activity of ATP-dependent malate lyase (acetylating CoA). ATP: malate lyase is supposed to be the specific enzyme of the cycle of the autotrophic CO2 fixation, in which pyruvate synthase, pyruvate phosphate dikinase, phosphoenolpyruvate (PEP) carboxylase and malate dehydrogenase are involved as well. The main product of the CO2 fixation cycle is glyoxylate, which could further be converted into 3-phosphoglyceric acid (3-PGA) in the reactions of either glycerate or serine pathway. The enzymes of both pathways were detected in C. auratiacus B-3. The results of the in vivo studies of glyxoylate and glycine metabolism, as well as the inhibitor analysis using fluoroacetate (FAc), isonicotinic acid hydrazide (INH), and 4-aminopterin (4-AP) confirm the operation of the proposed pathway in Chloroflexus.

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Abbreviations

3-PGA:

3-phosphoglyceric acid

4-AP:

4-aminopterin

FAc:

fluoroacetate

INH:

isonicotinic acid hydrazide

MV:

methyl viologen

PEP:

phosphoenolpyruvate

THF:

tetrahydrofolate

TPP:

thiamine pyrophosphate

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Authors and Affiliations

  1. Department of Microbiology, Moscow State University, 119899, Moscow, Russia

    Ruslan N. Ivanovsky, Elena N. Krasilnikova & Yuri I. Fal

Authors
  1. Ruslan N. Ivanovsky
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  2. Elena N. Krasilnikova
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  3. Yuri I. Fal
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Ivanovsky, R.N., Krasilnikova, E.N. & Fal, Y.I. A pathway of the autotrophic CO2 fixation in Chloroflexus aurantiacus . Arch. Microbiol. 159, 257–264 (1993). https://doi.org/10.1007/BF00248481

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  • DOI: https://doi.org/10.1007/BF00248481

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