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Herstellung eines Trockenpräparates alkylphosphatvergifteter Acetylcholinesterase für Reaktivierungsversuche

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Summary

An approach has been made to the problem of spontaneous recovery of acetylcholinesterase activity after alkylphosphate poisoning in vivo.

In order to get reliable measurements of rather small reactivation of acetylcholinesterase inhibited by alkylphosphates a procedure has been developed for the preparation of a soluble dry powder of pigs-brain which contains inhibited acetylcholinesterase. The overshoot of Paraoxon which did not react with the enzyme has been dialysed and adsorbed to active charcoal. It may be emphasized that the diminishing of reactivatability has been kept at a minimum during the time of preparation.

The presence of reactivating substances in serum of man, horse and mice has been reestablished and its occurrance in brain, liver and serum of rats has been shown. The compound is dialysable and stable against short heating to 95° C.

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Authors and Affiliations

  1. Aus dem Pharmakologischen Institut der Freien Universität Berlin, Germany

    H. Kewitz & V. Neuhoff

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  1. H. Kewitz
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  2. V. Neuhoff
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Stipendiat der Deutschen Forschungsgemeinschaft.

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Kewitz, H., Neuhoff, V. Herstellung eines Trockenpräparates alkylphosphatvergifteter Acetylcholinesterase für Reaktivierungsversuche. Naunyn - Schmiedebergs Arch 240, 126–133 (1960). https://doi.org/10.1007/BF00246616

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  • DOI: https://doi.org/10.1007/BF00246616

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