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Trennung und Charakterisierung der Abbauprodukte von DPN, DPNH und TPN nach enzymatischer Hydrolyse durch Gehirnhomogenate

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Summary

The degradation of DPN, TPN and DPNH by homogenates of brain has been studied. The products are separated and identified.

Nucleosidase of brain splits DPN in Nicotinamide and Adenosine-diphosphoribose (ARPPR). TPN has broken down to Nicotinamide and 2′-Phospho-adenosine-diphosphoribose \((A\mathop R\limits^P PPR)\) whereas DPNH is not metabolised by this enzyme.

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Literatur

  • Barnum, C. P., C. W. Nash, E. Jennings, O. Nygaard and H. Vermund: The Separation of Pentose and Desoxypentose nucleic acids from isolated mouse liver cell nuclei. Arch. Biochem. 25, 376 (1950).

    Google Scholar 

  • Berenblum, J., and E. Chain: An improved method for the colorimetric Determination of Phosphate. Biochem. J. 32, 295 (1938).

    Google Scholar 

  • Burton, R. M.: Alteration of Tissue Pyridine Nucleotide Levels and the Central Nervous System: Inhibition of the Nervous System and γ-Aminobutyric Acid, p. 249. Pergamon Press 1960.

  • Coper, H.: Der Einfluß verschiedener Pharmaka auf die Phosphorylierung von DPN zu TPN. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 236, 59 (1959).

    Google Scholar 

  • Eggleston, L. V., and R. Hems: Separation of Adenosine Phosphates by Paper-chromatography and the Equilibrium Constant of the Myokinase System. Biochem. J. 52, 156 (1952).

    Google Scholar 

  • Euler, H. v., F. Schlenk, H. Heiwinkel u. B. Högberg: Cocymase- und Nikotinsäureamidgehalt im Tierkörper und in der Hefe. Hoppe-Seylers Z. physiol. Chem. 256, 208 (1938).

    Google Scholar 

  • Forrest, R. J., D. R. Wilken and R. J. Hansen: The Presence of Adenosine-diphosphoribose and Adenosine-triphosphoribose in Acid Extracts of certain Animal Tissues. Biochem. biophys. Acta 37, 551 (1960).

    Google Scholar 

  • Handler, P., and J. R. Klein: The Inactivation of Pyridine Nucleotides by Animal Tissues in vitro. J. biol. Chem. 143, 49 (1942).

    Google Scholar 

  • Hurlbert, R. B., H. Schmitz, A. F. Brumm and V. J. Potter: Nucleotide Metabolism Chromatographic Separation of acidsoluble Nucleotides. J. biol. Chem. 209, 23 (1954).

    Google Scholar 

  • McIlwain, H.: Biochemistry and the Central Nervous System, p. 97. Little, Brown and Co. 1955.

  • McIlwain, H., and R. Rodnight: Breakdown of Cocymase by a System from nervous Tissue. Biochem. J. 44, 337 (1949a).

    Google Scholar 

  • McIlwain, H., and R. Rodnight: Breakdown of the oxidized Forms of Coenzyme I and II by an Enzyme from the Central Nervous System. Biochem. J. 45, 337 (1949b).

    Google Scholar 

  • Jacobson, K. B., and N. O. Kaplan: Distribution of Enzyme Cleaving Pyridine Nucleotides in Animal Tissues. J. biophys. biochem. Cytol. 3, 31 (1957).

    Google Scholar 

  • Kaplan, N. O.: Coenzyme Metabolism of the Brain: Brady and Tower, The Neurochemistry of Nucleotides and Amino-Acids, p. 870 (1960).

  • Kaplan, N. O., S. P. Colowick and A. Nason: Neurospora Diphosphopyridine Nucleotidase. J. biol. Chem. 191, 473 (1951).

    Google Scholar 

  • Koransky, W.: Trennung und Bestimmung der Nukleotide des Gehirns. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 234, 46 (1958).

    Google Scholar 

  • Kornberg, A.: Nucleotide Pyrophosphatase and Triphosphopyridine Nucleotide Structure. J. biol. Chem. 174, 1051 (1948).

    Google Scholar 

  • Kornberg, A., and O. Lindberg: Diphosphopyridine Nucleotide Pyrophosphatase. J. biol. Chem. 176, 665 (1948).

    Google Scholar 

  • Kornberg, A., and W. E. Pricer: Nucleotide Pyrophosphatase. J. biol. Chem. 182, 763 (1950).

    Google Scholar 

  • Lisboa, B. P.: Quantitative Bestimmung von Nikotinamid (NA) in Gegenwart von Nikotinsäure (NS). Naturwissenschaften 44, 617 (1957).

    Google Scholar 

  • Mann, P. J. G., and J. H. Quastel: Nicotinamide, Cocymase and Tissue Metabolism. Biochem. J. 35, 502 (1941).

    Google Scholar 

  • Mejbaum, W.: Über die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylsäure. Hoppe-Seylers Z. physiol. Chem. 258, 117 (1939).

    Google Scholar 

  • Morton, R. K.: The Action of Purified Alkaline Phosphatases on Di- and Tri-Phosphopyridine Nucleotides. Biochem. J. 61, 240 (1955).

    Google Scholar 

  • Neufeld, E. F., N. O. Kaplan and S. P. Colowick: Effect of Adenine Nucleotides on Reactions Involving Triphosphopyridine Nucleotide. Biochim. biophys. Acta 17, 525 (1955).

    Google Scholar 

  • Roberts, D. W. A.: The Hydrolysis of Diphosphopyridine Nucleotide by Juice expressed from Wheat Leaves. J. biol. Chem. 234, 655 (1959).

    Google Scholar 

  • Stepp, W., J. Kühnau u. H. Schröder: Die Vitamine und ihre klinische Anwendung. Stuttgart: Enke 1952.

    Google Scholar 

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  1. Aus dem Pharmakologischen Institut der Freien Universität Berlin, Germany

    H. Coper

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  1. H. Coper
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Coper, H. Trennung und Charakterisierung der Abbauprodukte von DPN, DPNH und TPN nach enzymatischer Hydrolyse durch Gehirnhomogenate. Naunyn - Schmiedebergs Arch 241, 335–345 (1961). https://doi.org/10.1007/BF00245067

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  • DOI: https://doi.org/10.1007/BF00245067

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