Summary
Plasma concentrations of atrial natriuretic peptide (ANP) were investigated daily in 16 male cadets during a 6-day military training course with continuous heavy physical activities, sleep and energy deficiency (course 1). At the end of another similar course (course 11) 15 cadets were studied during 30-min cycle exercise at 50% maximal oxygen uptake with and without glucose infusion. A small, but not significant increase was found in the plasma concentrations of ANP during course I from 9.6 (SEM 1.1) pmol·l−1 in the control experiment to 11.1 (SEM 0.5) pmol·l−1 on day 5. During course II a small but significant increase was found from 7.8 (SEM 0.5) pmol·l−1 in the control experiment to 9.1 (SEM 0.5) pmol·l−1 at the end of the course. Plasma osmolality and chloride concentration decreased during the course. During the exercise test a significant increase was seen in ANP concentration from 8.2 (SEM 0.8) to 13.1 (SEM 2.0) pmol·l−1 in the control experiment and from 9.4 (SEM 0.7) to 13.5 (SEM 1.2) pmol·l−1 during the course. This response was attenuated by glucose infusion, an effect which may have been due to an exercise induced increase in plasma chloride concentration being abolished. In contrast, the potassium concentration response to exercise was increased during the course but unaffected by glucose infusion. In conclusion, the large increases in endogenous plasma catecholamine concentration shown to take place during previous courses were not reflected in the plasma concentrations of ANP, indicating only a moderate cardiac stress or no cardiac work overload during such courses.
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Bahr R, Opstad PK, Medbø JI, Sejersted O (1991) Strenuous prolonged exercise elevates resting metabolic rate and causes reduced mechanical efficiency. Acta Physiol Scand 141:555–563
Christensen G, Ilebekk A, Aakeson I, Kiil F (1988) The release mechanism for atrial natriuretic factor during blood volume expansion and tachycardia in dogs. Acta Physiol Scand 134:263–270
Christensen G, Ilebekk A, Kiil F (1989) Release of atrial natriuretic factor during infusion of isoproterenol and angiotensin II. Am J Physiol 257:R896-R900
Christensen G, Aksnes G, Ilebekk A, Kiil F (1991) Release of atrial natriuretic factor during selective cardiac α- and β-adrenergic stimulation, intracoronary Ca2+ infusion, and aortic constriction in pigs. Circ Res 68:638–644
Clark BA, Sclater A, Epstein FH, Elahi D (1993) Effect of glucose, insulin, and hypertonicity on atrial natriuretic peptide concentrations in man. Metabolism 42:224–228
Currie MK, Newman WH (1986) Evidence for α1-adrenergic receptor regulation of atriopeptin release from the isolated rat heart. Biochem Biophys Res Commun 137:94–100
Kangawa K, Masuo H (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-ANP). Biochem Biophys Res Commun 118:131–139
Keller N, Møller T, Sykulski R, Storm TL, Thamsborg GM (1987) Effect of alfa-1 adrenoceptor blockade on plasma levels of atrial natriuretic peptide during dynamic exercise in normal man. Horm Metab Res 19:344
Lang RE, Thölken H, Ganten D, Luft FC, Ruskoaho H, Unger TH (1985) Atrial natriuretic factor — a circulating hormone stimulated by volume loading. Nature 314:264–266
Nakaoka H, Imataka K, Amano M, Fujii J, Ishibashi M, Yaaji T (1985) Plasma concentrations of atrial natriuretic factor in patients with congestive heart failure. N Engl J Med 313:892–893
Nilsson G, Pettersson A, Hedner J, Hedner T (1987) Increased plasma levels of atrial natriuretic peptide (ANP) in patients with paroxysmal supraventricular tachyarrhythmias. Acta Med Scand 221:15–21
Opstad PK (1990) Adrenergic desensitization and alteration in free and conjugated catecholamines during prolonged strain, sleep and energy deficieny. Biogenic Amines 6:625–639
Opstad PK, Aakvaag A, Rognum TO (1980) Altered hormonal response to short-term bicycle exercise in young men after prolonged physical strain, caloric deficit, and sleep deprivation. Eur J Appl Physiol 45:51–62
Opstad PK, Falch D, Øktedalen O, Fonnum F, Wergeland R (1984) The thyroid function in young men during prolonged exercise and the effect of energy and sleep deprivation. Clin Endocrinol 20:657–669
Opstad PK, Øktedalen O, Aakvaag A, Fonnum F, Lund PK (1985) Plasma renin activity and serum aldosterone during prolonged physical strain. The significance of sleep and energy deficiency. Eur J Appl Physiol 54:1–6
Opstad PK, Bråtveit M, Wiik P, Bøyum A (1944) The dynamic response of the β2- and α2-adrenoceptors in human blood cells to prolonged exhausting strain, sleep and energy deficiency. Biogenic Amines (in press)
Rashed HM, Nair BG, Patel TB (1992) Regulation of hepatic glycolysis and gluconeogenesis by atrial natriuretic peptide. Arch Biochem Biophys 298:640–645
Richards AM, Nicholls MG, Ikram H, Webster MWI, Yandle TG, Espiner EA (1985) Renal, haemodynamic, and hormonal effects of human alpha atrial natriuretic peptide in healthy volunteers. Lancet 1:545–549
Sanfield JA, Shenker Y, Grekin RJ, Rosen SG (1987) Epinephrine increases plasma immunoreactive atrial natriuretic hormone levels in humans. Am J Physiol 252:E740-E745
Shinoda T, Ishihara M, Kurimoto F, Aizawa T, Hiramatsu K, Shirota T, Takasu N, Yamada T (1990) Elevated plasma atrial natriuretic peptide level in the early phase of microalbuminuria in patients with non-insulin-dependent diabetes mellitus. Clin Nephrol 34:202–207
Sonnenberg H, Veress AT (1984) Cellular mechanism of release of atrial natriuretic factor. Biochem Biophys Res Commun 124:443–449
Tan ACITL, Kloppenborg PWC, Benraad TJ (1989) Influence of age, posture and intra-individual variation on plasma concentrations of atrial natriuretic peptide. Ann Clin Biochem 26:481–486
Taylor HL, Buskirk E, Henschel A (1955) Maximal oxygen intake as an objective measure of cardio-respiratory performance. J Appl Physiol 55:73–80
Thamsborg G, Storm T, Keller N, Sykulski R, Larsen J (1987) Changes in plasma atrial natriuretic peptide during exercise in healthy volunteers Acta Med Scand 221:441–444
Tikkanen I, Fyhrquist F, Metsärinne K, Leidenius R (1985) Plasma atrial natriuretic peptide in cardiac disease and during infusion in healthy volunteers. Lancet II:66–69
Tsai R-C, Yamaji T, Ishibashi M, Takaku F, Hsu S-T, Lai C-Y, Yeh S-J, Hung J-S, Wu D, Lee Y-S (1988) Effect of β-adrenergic blockade on plasma levels of atrial natriuretic peptide during exercise in humans. J Cardiovasc Pharm 11:614–618
Yamaji T, Ishibashi M, Nakaoka H, Imataka K, Amano M, Fujii J (1985) Possible role for atrial natriuretic peptide in polyuria associated with paroxysmal atrial arrhythmias. Lancet 1:1211
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Opstad, P.K., Haugen, A.H., Sejersted, O.M. et al. Atrial natriuretic peptide in plasma after prolonged physical strain, energy deficiency and sleep deprivation. Europ. J. Appl. Physiol. 68, 122–126 (1994). https://doi.org/10.1007/BF00244024
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DOI: https://doi.org/10.1007/BF00244024