Abstract
A procedure is described for standardising the determination of adenosine 5′-triphosphate and phosphocreatine concentration ([ATP] and [PC], respectively, in absolute arbitrary units) in human muscle by nuclear magnetic resonance (NMR) spectroscopy. The individual 31phosphorus (21P)-NMR spectra obtained on equal hemispherical tissue volumes (muscle plus skin and fat) were corrected for the thickness of the skin and of the subcutaneous fat. The volumes investigated were standardised using an external reference. The procedure described made possible the comparison of high energy phosphate concentrations among different subjects. It was applied to the assessment of [ATP] and [PC] in four groups of sedentary subjects (children, and adults aged 20–35, 35–50 and over 50 years), and in a group of athletes (volleyball players). The [ATP] and [PC] were not statistically different in the groups investigated.
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Arnold DL, Matthews PM, Radda GK (1984) Metabolic recovery after exercise and the assessment of mitochondrial function in vivo in human skeletal muscle by means of 31P-NMR. Magn Reson Med 1:307–315
Bergstrom J (1967)Local changes of ATP and PC in human muscle tissue in connection with exercise. Circ Res 20 [Suppl 1]: 91–96
Binzoni T, Ferretti G, Schenker K, Cerretelli P (1992) Phosphocreatine hydrolysis by 31P-NMR at the onset of constant-load exercise in humans. J Appl Physiol 73:1644–1649
Chance B, Eleff S, Leigh JS, Sokolow D, Sapega A (1981) Mitochondrial regulation of phosphocreatine/inorganic phosphate ratios in exercising human muscle: a gated 31P-NMR study. Proc Natl Acad Sci USA 78:6714–6718
Chance B, Leigh S, Clark FJ, Maris J, Kent J, Nioka S, Smith D (1985) Control of oxidative metabolism and oxygen delivery in human skeletal muscle: a steady-state analysis of the work/energy cost transfer function. Proc Natl Acad Sci USA 82:8384–8388
Chance B, Leigh S, Kent J, McCully K, Niokas S, Clark BJ, Maris J, Graham T (1986) Control of oxidative metabolism and oxygen delivery in human skeletal muscle: multiple controls of oxidative metabolism in living tissues as studied by phosphorus magnetic resonance. Proc Natl Acad Sci USA 83:9458–9462
Cheetham ME, Boobis LH, Brooks S, Williams C (1986) Human muscle metabolism during sprint running. J Appl Physiol 61:54–60
Decorps M, Blondet P, Reutenauer H, Albrand JP, Remy C (1985) An inductively coupled, series-tuned probe. J Magn Res 65:100–109
Eriksson B (1980) Muscle metabolism in children: a review. Acta Physiol Scand 283:20–27
Ferretti G, Narici MV, Binzoni T, Gariod L, Le Bas JF, Reutenauer H, Cerretelli P (1994) Determinants of peak muscle power. Effects of age and physical conditioning. Eur J Appl Physiol (in press)
Green HJ, Sutton J, Young P, Cymerman A, Houston CS (1989) Operation Everest II: muscle energetics during maximal exhaustive exercise. J Appl Physiol 66:142–150
Harris RC, Sahlin K, Hultman E (1977) Phosphagen and lactate contents of m quadriceps femoris of man after exercise. J Appl Physiol 43:852–857
Jones NL, McCarney N, Graham T, Spriet LL, Kowalchuk JM, Heigenhauser GJF, Sutton JR (1985) Muscle performance and metabolism in maximal isokinetic cycling at slow and fast speeds. J Appl Physiol 59:132–136
Karlsson J, Saltin B (1970) Lactate, ATP, and CP in working muscles during exhaustive exercise in man. J Appl Physiol 29:598–602
Karlsson J, Nordesjo LO, Jorfeldt L, Saltin B (1972) Muscle lactate, ATP, and CP levels during exercise after physical training. J Appl Physiol 33:199–203
Larsson LL, Sjodin B, Karlsson J (1978) Histochemical and biochemical changes in human skeletal muscle with age in sedentary males, age 22–65 years. Acta Physiol Scand 103:31–39
Laurent D, Authier B, Lebas JF, Rossi A (1992) Effect of prior exercise in Pi/PC ratio and pH during a standardized exercise. A study on human muscle using [31P]NMR. Acta Physiol Scand 144:31–38
McCully KK, Kent JA, Chance B (1988) Application of 31P magnetic resonance spectroscopy to the study of athletic performance. Sports Med 5:312–321
Meyer RA (1988) A linear model of muscle respiration explains monoexponential phosphocreatine changes. Am J Physiol 254:C548-C553
Meyer RA (1989) Linear dependence of muscle phosphocreatine kinetics on total creatine content. Am J Physiol 257: C1149-C1157
Meyer RA, Brown TR, Kushmerick MJ (1985) Phosphorus nuclear magnetic resonance of fast- and slow-twitch muscle. Am J Physiol 248:C279-C287
Miller RG, Boska MD, Moussavi RS, Carson PJ, Weiner MW (1988) 31P nuclear magnetic resonance studies of high energy phosphates and pH in human muscle fatigue. J Clin Invest 81:1190–1196
Mold PA, Coulson RL, Caton JR, Nichols BG, Barstow TJ (1985) In vivo 31P-NMR in human muscle: transient patterns with exercise. J Appl Physiol 59:101–104
Saltin B, Gollnick PD (1983) Skeletal muscle adaptability: significance for metabolism and performance. In: Peachey CD, Adrian RH, Geiger SR (eds) Handbook of physiology. Skeletal muscle. American Physiological Society, Bethesda, Md, pp 555–631
Taylor DJ, Bore PJ, Styles P, Gadian DG, Radda GK (1983) Bioenergetics of intact human muscle. A 31P nuclear magnetic resonance study. Mol Biol Med 1:77–94
Thorstensson A, Sjodin B, Karlsson J (1975). Enzyme activities and muscle strength after sprint training in man. Acta Physiol Scand 94:313–318
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Gariod, L., Binzoni, T., Ferretti, G. et al. Standardisation of 31phosphorus-nuclear magnetic resonance spectroscopy determinations of high energy phosphates in humans. Europ. J. Appl. Physiol. 68, 107–110 (1994). https://doi.org/10.1007/BF00244021
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DOI: https://doi.org/10.1007/BF00244021