Abstract
The quantity and quality of light required for light-stimulated cell expansion in leaves of Phaseolus vulgaris L. have been determined. Seedlings were grown in dim red light (RL; 4 μmol photons·m−2·s−1) until cell division in the primary leaves was completed, then excised discs were incubated in 10 mM sucrose plus 10 mM KCl in a variety of light treatments. The growth response of discs exposed to continuous white light (WL) for 16 h was saturated at 100 μmol·m−-2·s−1, and did not show reciprocity. Extensive, but not continuous, illumination was needed for maximal growth. The wave-length dependence of disc expansion was determined from fluence-response curves obtained from 380 to 730 nm provided by the Okazaki Large Spectrograph. Blue (BL; 460 nm) and red light (RL; 660 nm) were most effective in promoting leaf cell growth, both in photosynthetically active and inhibited leaf discs. Farred light (FR; 730 nm) reduced the effectiveness of RL, but not BL, indicating that phytochrome and a separate blue-light receptor mediate expansion of leaf cells.
Similar content being viewed by others
Abbreviations
- BL:
-
blue light
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- FR:
-
far-red light
- RL:
-
red light
- WL:
-
white light
References
Assmann, S.M., Simoncini, L., Schroeder, J.I. (1985) Blue light activates electrogenic ion pumping in guard cell protoplasts of Vicia faba L. Nature 318, 285–287
Butler, R.D. (1963) The effect of light intensity on stem and leaf growth in broad bean seedlings. J. Exp. Bot. 14, 142–152
Dale, J.E. (1965) Leaf growth in Phaseolus. 2. Temperature effects and the light factor. Ann. Bot. 29, 293–308
Dale, J.E. (1988) The control of leaf expansion. Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 267–295
Dale, J.E., Murray, D. (1968) Photomorphogenesis, photosynthesis and early growth of primary leaves of Phaseolus vulgaris. Ann. Bot. 32, 767–780
de Greef, J.A., Caubergs, R., Verbelen, J.P., Moereels, E. (1978) Phytochrome-mediated inter-organ dependence and rapid transmission of the light stimulus. In: Light and plant development, pp. 295–315, Smith, H.H., ed. Butterworths, London
Göring, H., Koshuchowa, S., Munnich, H., Dietrich, M. (1985) Stomatal opening and cell enlargement in response to light and phytohormone treatments in primary leaves of red-light-grown seedlings of Phaseolus vulgaris L. Plant Cell Physiol. 25, 683–690
Loach, K. (1970) Shade tolerance in tree seedlings. II. Growth analysis of plants raised under artificial shade. New Phytol. 69, 273–286
Milthorpe, F.L., Newton, P. (1963) Studies on the expansion of the leaf surface. III. The influence of radiation on cell division and leaf expansion. J. Exp. Bot. 14, 483–495
Mohr, H. (1959) Der Lichteinfluss auf das Wachstum der Keimblätter bei Sinapis alba L. Planta 53, 219–245
Prins, H.B.A. (1970) The effect of DCMU on ion uptake and photosynthesis in leaves of Vallisneria spiralis L. Acta. Bot. Neerl. 19, 813–820
Sale, P.J.M., Vince, D. (1963) Some effects of light on leaf growth in Pisum sativum and Tropaeolum majus. Photochem. Photobiol. 2, 401–405
Serrano, E.E., Zeiger, E., Hagiwara, S. (1988) Red light stimulates an electrogenic proton pump in Vicia guard cell protoplasts. Proc. Natl. Acad. Sci. USA 85, 463–440
Van Volkenburgh, E., Cleland, R.E. (1979) Separation of cell enlargement and division in bean leaves. Planta 146, 245–247
Van Volkenburgh, E., Cleland, R.E. (1980) Proton excretion and cell expansion in bean leaves. Planta 148, 273–278
Van Volkenburgh, E., Cleland, R.E. (1990) Light-stimulated cell expansion in bean (Phaseolus vulgaris L.) leaves. I. Growth can occur without photosynthesis. Planta 182, 72–76
Verbelen, J.P., de Greef, J.A. (1979) Leaf development of Phaseolus vulgaris L. in light and darkness. Am. J. Bot. 66, 970–976
Vince-Prue, D., Tucker, D.J. (1982) Photomorphogenesis in leaves. In: The growth and functioning of leaves, pp. 233–269, Dale, J.E., Milthorpe, F.L., eds. Cambridge University Press, Cambridge, UK
Watanabe, M., Furuya, M., Miyoshi, Y., Inoue, Y., Iwahashi, I., Matsumoto, K. (1982) Design and performance of the Okazaki large spectrograph for photobiological research. Photochem. Photobiol. 36, 491–498
Author information
Authors and Affiliations
Additional information
We wish to thank Professor M. Furuya for inviting E.V. to Okazaki, and B. Lippitt, M. Kubota, and J. Roden for their invaluable, expert technical assistance. We also thank Dr. K.T. Yamamoto for planting and caring for the plants prior to experimentation in Okazaki. This study was carried out under the NIBB Cooperative Research Program for the Okazaki Large Spectrograph (project no. 86-513).
Rights and permissions
About this article
Cite this article
van Volkenburgh, E., Cleland, R.E. & Watanabe, M. Light-stimulated cell expansion in bean (Phaseolus vulgaris L.) leaves. Planta 182, 77–80 (1990). https://doi.org/10.1007/BF00239987
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00239987