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Pigment-dispersing hormone-immunoreactive fibers persist in crickets which remain rhythmic after bilateral transection of the optic stalks

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Abstract

Lesion studies combined with immunocytochemical experiments were used to examine whether pigment-dispersing hormone-immunoreactive processes in the midbrain of crickets correlate with the presence of circadian activity. After interruption of both optic stalks in the crickets Teleogryllus commodus and Gryllus bimaculatus animals retained circadian rhythms in their activity patterns. This suggests that there is another circadian oscillator in the midbrain. The pigment-dispersing hormone-immunoreactive fibers in the midbrain of both operated species appeared not to degenerate although they were separated from their somata in the optic lobes. These data could suggest that surviving fibers, separated from the somata of the circadian pacemaker in the optic lobes, contribute to the persistance of circadian activity in operated crickets.

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Abbreviations

DD :

constant darkness

LD :

light dark cycles

PDH :

pigment-dispersing hormone

PDHLI:

pigment-dispersing hormone-like immunoreactivity

PDH Me :

pigment-dispersing hormone-immunoreactive cells of the accessory medulla

QV :

Quantification value

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  1. Institut für Zoologie, Universität Regensburg, Universitätsstrasse 31, D-93053, Regensburg, Germany

    M. Stengl

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Stengl, M. Pigment-dispersing hormone-immunoreactive fibers persist in crickets which remain rhythmic after bilateral transection of the optic stalks. J Comp Physiol A 176, 217–228 (1995). https://doi.org/10.1007/BF00239924

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