Summary
Induction of somatic embryogenesis by different growth regulators was examined in leaf disc cultures of Nicotiana tabacum L. Direct differentiation of somatic embryos occurred on media supplemented with naphthaleneacetic acid (NAA) and N6-benzylaminopurine (BAP). Thidiazuron (N-phenyl-N′-1, 2,3,-thiadiazol-5-ylurea; TDZ) not only substituted for the most effective NAA-BAP combination but also induced a higher frequency of somatic embryogenesis. Regenerated somatic embryos were capable of developing into plants.
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Abbreviations
- BAP:
-
N6-benzylaminopurine
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
Naphthaleneacetic acid
- TDZ:
-
N-phenyl-N′-1,2,3,- thiadiazol-5-ylurea
References
Arndt F, Rusch R, Stillfried HV (1976) SN 49537, a new cotton defoliant. Plant Physiol. 57: 99.
Brown DCW, Thorpe TA (1986) Plant regeneration by organogenesis. In: Cell culture and somatic cell genetics of plants (IK Vasil ed.) pp. 49–65. Academic Press. Orlando, Florida.
Capelle SC, Mok DWS, Sandra C, Kirchner SC, Mok MC (1983) Effect of thidiazuron on cytokinin autonomy and the metabolism of N6-(Δ2-Isopentenyl) [8-14C] adenosine in callus tissue of Phaseolus lunatus. Plant Physiol. 73: 796–802.
Gill R, Saxena PK (1992) Direct somatic embryogenesis and regeneration of plants from seedling expiants of peanut (Arachis hypogaea L): Promotive role of thidiazuron. Can. J. Bot. 70: 1186–1192.
Haccius B, Lakshmanan KK (1965) Adventive embryony in callus culture of Nicotiana under high light intensity. Planta 65: 102–104.
Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L. High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186: 384–389.
Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okanoto T (1982) Cytokinin activity of N-phenyl-N′-1,2,3-thidiazol-5 yl urea (thidiazuron). Phytochemistry 21: 1509–1511.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 473–497.
Prabhudesat VR, Narayanswamy, S (1974) Differentiation of cytokinininduced shoot buds and embryoids on excised petioles of Nicotiana tabacum. Phytomorphology 23: 133–137.
Saxena PK, Malik KA, Gill R (1992) Induction by thidiazuron of direct somatic embryogenesis in intact seedlings of peanut. Planta 187: 421–424.
Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–140.
Stolarz A, Macewicz J, Lorz H (1991) Direct somatic embryogenesis and plant regeneration from leaf expiants of Nicotiana tabacum L. J. Plant Physiol. 137: 347–357.
Thomas JC, Katterman FR (1986) Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681–683.
Van Nieuwkerk, Zimmerman RH, Fordham I (1986). Thidiazuron stimulation of apple shoot proliferation in vitro. Horticulture Sci. 21: 516–518.
Visser C, Qureshi JA, Gill R and Saxena PK (1992) Morphoregulatory role of thidiazuron. Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol. 99: 1704–1707.
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Gill, R., Saxena, P.K. Somatic embryogenesis in Nicotiana tabacum L.: induction by thidiazuron of direct embryo differentiation from cultured leaf discs. Plant Cell Reports 12, 154–159 (1993). https://doi.org/10.1007/BF00239097
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DOI: https://doi.org/10.1007/BF00239097