Abstract
Embryonal-suspensor mass lines (ESM lines) from mature embryos of three open-pollinated families of Norway spruce [Picea abies (L.) Karst.] were stabilized on several culture media varying in nutrient composition. A pattern of relationship between parameters of polyembryogenic culture performance and medium composition was genetically determined. Stabilized ESM lines could be classified into two phenovariants: (i) lines of homogenous ESM composed entirely of somatic proembryos, and (ii) lines of heterogeneous ESM composed of a mixture of nonembryogenic callus and aggregates of somatic proembryos. The formation of one or the other phenovariant was independent of genotype, but strongly determined by the medium composition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA:
-
abscisic acid
- BA:
-
6-benzyladenine
- ESM:
-
embryonal-suspensor mass
- N:
-
nitrogen
- NAA:
-
α-naphthyl-1-acetic acid
References
Bozhkov PV, Lebedenko LA, Shiryaeva GA (1992) Plant Cell Rep. 11: 386–389
Bozhkov PV, Lebedenko LA, Shiryaeva GA (1993a) Russia Patent 1761057
Bozhkov PV, Mikhlina SB, Shiryaeva GA, Lebedenko LA (1993b) J. Plant Physiol. 142: 735–741
Braun AC, Meins F Jr (1970) Symp. Int. Soc. Cell Biol. 9: 193–204
Dong J, Wagner DB, Yanchuk AD, Carlson MR, Magnussen S, Wang X-R, Szmidt AE (1992) J. Heredity 83: 419–433
Durzan DJ (1988) Biotechnology Genet. Eng. Revs. 6: 341–378
Durzan DJ (1989) Ann. Sci. For. 46 suppl.: 101s–107s
Gelfant S, Smith JG (1972) Science 178: 357–361
Klimaszewska K (1989) Plant Sci. 63: 95–103
Krogstrup P (1986) Can. J. For. Res. 16: 664–668
Litvay JD, Johnson MA, Verma D, Einspahr D, Weyrauch K (1981) Institute Paper Chemistry Technical Paper Series 115: 1–17
Roberts DR, Webster FB, Flinn BS, Lararoff WR, Cyr DR (1993) In: Redenbaugh K (ed) Synseeds: application of synthetic seeds to crop improvement, CRC Press, Boca Raton, pp 427–449
Sagi L, Barnabas B (1989) Theor. Appl. Genet. 78: 867–872
Tautorus TE, Attree SM, Fowke LC, Dunstan DI (1990) Plant Sci. 67: 115–124
von Arnold S, Eriksson T (1981) Can. J. Bot. 59: 870–874
Wann SR, Johnson MA, Noland TL, Carlson JA (19873) Plant Cell Rep. 6: 39–42
Webb DT, Webster F, Flinn BS, Roberts DR, Ellis DD (1989) Can. J. For. Res. 19: 1303–1308
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Bozkov, P.V. Genotypic and media factors affecting stabilization of polyembryogenic cultures in norway spruce (Picea abies (L.) Karst.). Plant Cell Reports 14, 389–392 (1995). https://doi.org/10.1007/BF00238603
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00238603