Abstract
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.
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Abbreviations
- BA:
-
6-benzylaminopurine
- GA3 :
-
Gibberellic acid
- IBA:
-
indole-3-butyric acid
- IAA:
-
indole-3-acetic acid
- NAA:
-
naphthalene acetic acid
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Watad, A.A., Kochba, M., Nissim, A. et al. Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts. Plant Cell Reports 14, 345–348 (1995). https://doi.org/10.1007/BF00238594
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DOI: https://doi.org/10.1007/BF00238594