Summary
Light and electron microscope autoradiography indicated that 3H-labelled inulin was taken up by neurons and glia cells of rat brain cortex in vitro.
The mechanism, by which inulin passed the cell membranes, was studied by comparing the transport of inulin (molecular weight 5000) with the transport of dextran (molecular weight 75000). The half-time for the cellular in- and efflux for the two molecules was the same although their diffusion coefficients differed by a factor of 4–5. The transport mechanism was therefore interpreted as bulk transport, and vesicular transport is suggested.
Efflux of inulin from brain cortex exposed to inulin in vivo indicated that cellular uptake of inulin also occured in vivo.
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Andres, K.H.: Mikropinocytose im Zentralnervensystem. Z. Zellforsch. 64, 63–73 (1964)
Bondareff, W.: Distribution of ferritin in cerebral cortex of the mouse revealed by electron microscopy. Exp. Neurol. 10, 377–382 (1964)
Brightman, M.W.: The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. II. Parenchymal distribution. Amer. J. Anat. 117, 193–220 (1965)
Brodal, A.: Neurological Anatomy, pp. 654–655. London: Oxford University Press 1969
Brown, D.A., Stumpf, W.E., Diab, I.M., Roth, L.J.: Assessment of autoradiographic resolution using soluble extracellular fluid indicators. In: Autoradiography of diffusible substances (eds. L.J. Roth and W.E. Stumpf), pp. 161–182. New York: Academic Press 1969
Cerro, del M.: Uptake of tracer proteins in developing cerebellum particularly by growth cones and blood vessels. J. comp. Neurol. 157, 245–280 (1974)
Cohen, S.R.: A rapid, sensitive, semimicro gelfiltration procedure for detecting and removing low molecular weight fragments from [3H]- or [14C]-labelled inulin. Analyt. Biochem. 31, 539–544 (1969)
Granath, K.A., Kvist, B.E.: Molecular weight distribution analysis by gel chromatography on sephadex. J. Chromatog. 28, 69–81 (1967)
Klatzo, L., Miguel, J.: Observations on pinocytosis in nervous tissue. J. Neuropath. exp. Neurol. 19, 475–487 (1960)
Levin, E., Sisson, W.B.: The penetration of radiolabelled substances into rabbit brain from subarachnoid space. Brain Res. 41, 145–153 (1972)
Lund-Andersen, H.: Extracellular and intracellular distribution of inulin in rat brain cortex slices. Brain Res. 65, 239–254 (1974)
Møller, M., Lund-Andersen, H.: Autoradiography of tritiated inulin in epon imbedded rat brain cortex slices. Proc. roy. Microsco. Soc. 10, 158–159 (1975)
Møller, M., Møllgård, K., Lund-Andersen, H., Hertz, L.: Concordance between morphological and biochemical estimates of fluid spaces in rat brain cortex slices. Exp. Brain Res. 22, 299–314 (1974)
Nagasawa, J., Douglas, W.W., Schulz, R.A.: Micropinocytotic origin of coated and smooth microvesicles (“synaptic vesicles”) in neurosecretory terminals of posterior pituitary glands demonstrated by incorporation of horseradish peroxidase. Nature (Lond.) 232, 341–342 (1971)
Nicholls, J.B., Wolfe, D.E.: Distribution of [14C]-labelled sucrose, inulin and dextran in extracellular spaces and in cells of the leech central nervous system. J. Neurophysiol. 30, 1574–1592 (1967)
Pearse, A.G.E.: Histrochemistry, theoretical and applied. 3. ed. Vol. 1. London: Churchill 1969
Turner, P.T., Harris, A.B.: Ultrastructure of exogenous peroxidase in cerebral cortex. Brain Res. 74, 305–326 (1974)
Vise, W.M., Liss, L., Yashon, D., Hunt, W.E.: Astrocytic processes. A route between vessels and neurons following blood-brain barrier injury. J. Neuropath, exp. Neurol. 34, 324–334 (1975)
Weldon, P.R.: Pinocytotic uptake and intracellular distribution of colloidal thorium dioxide by cultured sensory neurites. J. Neurocytol. 4, 341–356 (1975)
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Lund-Andersen, H., Møller, M. Uptake of inulin by cells in rat brain cortex. Exp Brain Res 28, 37–50 (1977). https://doi.org/10.1007/BF00237084
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DOI: https://doi.org/10.1007/BF00237084