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Micropropagation of meadowfoam (Limnanthes spp.)

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Summary

A rapid micropropagation system was developed for meadowfoam (Limnanthes spp. Brown) using four genotypes of three species. Murashige and Skoog (MS) medium supplemented with N6 benzyladenine (BA) and indole-3-acetic acid (IAA) at 0, 0.1, 0.5, 1.0 and 2.0 mg/l was tested for multiplication, shoot elongation and rooting. Expiants were taken from pot-grown plants. The most useful level for shoot growth and multiplication of both floral induced and non-induced plants was 0.5 mg/l BA. IAA failed to affect shoot growth or multiplication. Expiants from non-induced plants multiplied at moderate to high rates on 0.5 mg/l BA, while those from induced plants multiplied slowly and tended to elongate and flower. Non-induced plants on 2 mg/l BA produced large numbers of tiny shoots; induced plants did not respond. Shoots of all genotypes rooted on MS medium without hormones and all plants grew normally after transplanting to soil. This system provides a new tool for the development of meadowfoam as a crop plant.

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Abbreviations

(BA):

N6-benzyladenine

(IAA):

indole-3-acetic acid

(MS):

Murashige and Skoog medium, 1962

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Authors and Affiliations

  1. USDA/ARS National Clonal Germplasm Repository, 33447 Peoria Road, 97333, Corvallis, OR, USA

    Barbara M. Reed

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  1. Barbara M. Reed
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Communicated by G. C. Phillips

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Reed, B.M. Micropropagation of meadowfoam (Limnanthes spp.). Plant Cell Reports 10, 94–96 (1991). https://doi.org/10.1007/BF00236465

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  • DOI: https://doi.org/10.1007/BF00236465

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