Abstract
In vitro clonai multip1ication of Coleus forskahlii Briq a threatened plant, has been achieved on MS medium supplemented with Kn (2.0 mg/l) and IAA (1.0 mg/l) using nodal segments as explants, Shoots multiplied at a rate of 12 — fold every six weeks. Rooting was achieved upon transfer of shoots onto MS medium containing IAA (1.0 mg/l). The micropropagated plants were successfully established under field conditions. Forskolin content in tubers of plants obtained by micropropagation was found to be 0.1%, the same as that found in wild plants. This micropropagation procedure should be useful for conservation as well as production of this important plant
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- Kn:
-
Kinetin
- MS:
-
Murashige and Skoog (1962) basal medium
- NAA:
-
∝-naphthalene acetic acid
References
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Communicated by G. C. Phillips
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Sharma, N., Chandel, K.P.S. & Srivastava, V.K. In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Reports 10, 67–70 (1991). https://doi.org/10.1007/BF00236459
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DOI: https://doi.org/10.1007/BF00236459