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Metabolism of glycoconjugates in hypothalamic neurons and glial cells: Comparison of incorporation of [3H]fucose and [3H]N-acetylmannosamine by electron microscopic autoradiography

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Summary

Incorporation of two glycoconjugate precursors, [3H]fucose and [3H]N-acetylmannosamine, in the arcuate nucleus of the rat hypothalamus is compared 30 min after intraventricular administration. Electron microscopic autoradiographs were analyzed by a sampling technique which provides information about (i) the distribution of radioactivity in the tissue, (ii) the relative volumes, and (iii) the amount of radioactivity per unit volume of the various tissue compartments. With both precursors, the highest incorporation was found in glial cell bodies, being about five to six times that of neurons. This holds true for all three types of glia. The concentration of radioactivity in the neuropil consisting of neuronal and glial processes was exceedingly low, although, due to its large volume, it contained the highest fraction of total tissue radioactivity. The limitations imposed on the interpretation of the data in terms of synthesis of fucosyland sialoglycoconjugates are discussed. It is proposed that the observations support the concept that high rates of synthesis for glycoproteins (and possibly gangliosides) are a characteristic metabolic property of glial cells.

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References

  • Brunngraber, E.G.: Chemistry and metabolism of glycopeptides derived from brain glycoproteins. In: Glycolipids, glycoproteins, and mucopolysaccharides of the nervous system (V. Zambotti, G. Tettamanti and M. Arrigoni, eds.), pp. 17–49. New York-London: Plenum Press 1972

    Google Scholar 

  • Changaris, D.G., Schengrund, C.-L., Combs, J.W.: Microfluorimetry of brain glycolipid. Theoretical and applied analysis of PAS fluorescence and dye concentration. J. Histochem. Cytochem. 26, 267–276 (1978)

    Google Scholar 

  • Cremer, J.E., Johnston, P.V., Roots, B.I., Trevor, A.J.: Heterogeneity of brain fractions containing neuronal and glial cells. J. Neurochem. 15, 1361–1370 (1968)

    Google Scholar 

  • Hamberger, A., Svennerholm, L.: Composition of gangliosides and phospholipids of neuronal and glial cell enriched fractions. J. Neurochem. 18, 1821–1829 (1971)

    Google Scholar 

  • Henn, F.A., Anderson, D.J., Rustad, D.G.: Glial contamination of synaptosomal fractions. Brain Res. 101, 341–344 (1976)

    Google Scholar 

  • Johnston, P.V., Roots, B.I.: Neuronal and glial perikarya preparations: an appraisal of present methods. Int. Rev. Cytol. 29, 265–280 (1970)

    Google Scholar 

  • Jones, J.P., Ramsey, R.B., Aexel, R.T., Nicholas, H.J.: Lipid biosynthesis in neuron-enriched and glialenriched fractions of rat brain: gangl-ioside biosynthesis. Life Sci. 11, 309–315 (1972)

    Google Scholar 

  • Langer, G.A., Frank, J.S., Nudd, L.M., Seraydarian, K.: Sialic acid: effect of removal on calcium exchangeability of cultured heart cells. Science 193, 1013–1015 (1976)

    Google Scholar 

  • Margolis, R.K., Margolis, R.U.: Disposition of fucose in brain. J. Neurochem. 19, 1023–1030 (1972)

    Google Scholar 

  • Margolis, R.U., Margolis, R.K.: Distribution and metabolism of mucopolysaccharides and glycoproteins in neuronal perikarya, astrocytes, and oligodendroglia. Biochemistry 13, 2849–2852 (1974)

    Google Scholar 

  • Morgan, I.G.: Synaptosomes and cell separation. Neuroscience 1, 159–165 (1976)

    Google Scholar 

  • Pilgrim, Ch., Wagner, H.-J.: Glycoprotein metabolism in the hypothalamus of rat: significance of glial cells. Histochemistry 45, 289–297 (1975)

    Google Scholar 

  • Poduslo, S.E., Norton, W.T.: Isolation and some chemical properties of oligodendroglia from calf brain. J. Neurochem. 19, 727–736 (1972)

    Google Scholar 

  • Quarles, R.H., Brady, R.O.: Synthesis of glycoproteins and gangliosides in developing rat brain. J. Neurochem. 18, 1809–1820 (1971)

    Google Scholar 

  • Radin, N.S., Brenkert, A., Arora, R.C., Sellinger, O.Z., Flangas, A.L.: Glial and neuronal localization of cerebroside-metabolizing enzymes. Brain Res. 39, 163–169 (1972)

    Google Scholar 

  • Rahmann, H., Rösner, H., Breer, H.: A functional model of sialoglyco-macromolecules in synaptic transmission and memory formation. J. Theor. Biol. 57, 231–237 (1976)

    Google Scholar 

  • Reisert, I., Pilgrim, Ch., Venedey, Ch.: Incorporation of [3H]leucine into hypothalamic nerve and glial cells. A comparison by EM autoradiography. Brain Res. (in press, 1979)

  • Reisert, I., Wagner, H.-J., Pilgrim, Ch.: Incorporation of [3H]fucose into nerve and glial cells: assessment by electron microscopic autoradiography. J. Comp. Neurol. 176, 453–466 (1977)

    Google Scholar 

  • Rösner, H., Wiegandt, H., Rahmann, H.: Sialic acid incorporation into gangliosides and glycoproteins of the fish brain. J. Neurochem. 21, 655–665 (1973)

    Google Scholar 

  • Rohr, H., Oberholzer, M., Bartsch, G., Keller, M.: Morphometry in experimental pathology: Methods, baseline data, and applications. In: International Review of Experimental Pathology, Vol. 15 (G.W. Richter and M.A. Epstein, eds.), pp. 233–325. New York: Academic Press 1976

    Google Scholar 

  • Salpeter, M.M., Bachmann, L., Salpeter, E.E.: Resolution in electron microscope radioautography. J. Cell Biol. 41, 1–20 (1969)

    Google Scholar 

  • Stoolmiller, A.C., Dawson, G., Dorfman, A.: The metabolism of glycosphingolipids and glycosaminoglycans. In: Tissue Culture of the Nervous System (G. Sato, ed.), pp. 247–280. New York-London: Plenum Press 1973

    Google Scholar 

  • Wagner, H.-J., Reisert, I., Möhler, E., Pilgrim, Ch.: Überexposition und Blockkontrastierung als Fehlerquellen der quantitativen Auswertung von elektronenmikroskopischen Autoradiogrammen. Acta Histochem. Suppl. XX (im Druck) (1978)

  • Williams, M.A.: The assessment of electron microscopic autoradiographs. Advan. Opt. Elect. Microsc. 3, 219–272 (1969)

    Google Scholar 

  • Wooley, D.W., Gommi, B.W.: Serotonin receptors, VII. Activities of various pure gangliosides as the receptors. Proc. Nat. Acad. Sci. USA 53, 959–963 (1965)

    Google Scholar 

  • Yohe, H.C., Rosenberg, A.: Action of intrinsic sialidase of rat brain synaptic membranes on membrane sialolipid and sialoprotein components in situ. J. Biol. Chem. 252, 2412–2418 (1977)

    Google Scholar 

  • Zatz, M., Barondes, S.H.: Fucose incorporation into glycoproteins of mouse brain. J. Neurochem. 17, 157–163 (1970)

    Google Scholar 

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  1. Abteilung für Klinische Morphologie der Universität Ulm, Bundesrepublik Deutschland

    I. Reisert & Ch. Pilgrim

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  1. I. Reisert
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  2. Ch. Pilgrim
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Dedicated to Prof. Emil Tonutti on the occasion of his 70th birthday

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Reisert, I., Pilgrim, C. Metabolism of glycoconjugates in hypothalamic neurons and glial cells: Comparison of incorporation of [3H]fucose and [3H]N-acetylmannosamine by electron microscopic autoradiography. Cell Tissue Res. 196, 135–145 (1979). https://doi.org/10.1007/BF00236354

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  • DOI: https://doi.org/10.1007/BF00236354

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