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Micropropagation of Morus laevigata Wall. from mature trees

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Abstract

Multiple shoots were obtained from nodal explants of 10-year-old tree of Morus laevigata on Murashige and Skoog's medium supplemented with different concentrations (0.5–5.0 mg.l−1) of benzyladenine (BA). Nodal segments taken from in vitro proliferated shoots gave further multiple shoots when cultured on the same basal medium containing 2.5 mg.l−1 BA. Repeated subculture resulted in rapid shoot multiplication at the average rate of 6-fold per subculture. In vitro raised shoots rooted on MS medium containing 0.1 mg. l−1 each of 3-indolebutyric acid (ISA) and α-naphthaleneacetic acid (NAA). The regenerated plantlets were successfully established in soil under field conditions after a few days of indoor acclimatization.

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Authors and Affiliations

  1. Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh

    M. Hossain, S. M. Rahman, A. Zaman, O. I. Joarder & R. Islam

Authors
  1. M. Hossain
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  2. S. M. Rahman
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  3. A. Zaman
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  4. O. I. Joarder
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  5. R. Islam
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Communicated by G.C. Phillips

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Hossain, M., Rahman, S.M., Zaman, A. et al. Micropropagation of Morus laevigata Wall. from mature trees. Plant Cell Reports 11, 522–524 (1992). https://doi.org/10.1007/BF00236269

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  • DOI: https://doi.org/10.1007/BF00236269

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