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Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum

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Abstract

Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro.

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References

  • Arcioni S, Mariotti D, Pezzotti M (1985) J. Plant Physiol. 121:141–148

    Google Scholar 

  • Arcioni S, Mariotti D, Damiani F, Pezzotti M (1988) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry Vol 6, Springer-Verlag, Berlin, pp 548–572

    Google Scholar 

  • Bovo OA, Mroginski LA, Rey HY (1986) Plant Cell Reports 5:295–297

    Google Scholar 

  • Clements RJ, Williams RJ (1980) In: Summerfield RJ, Bunting AH (eds) Advances in Legume Science, Royal Botanic Gardens Kew, England, pp 559–567

    Google Scholar 

  • Fernández JG, Benites CA, Pizzio RM, Royo Pallarés O (1988) Serie Técnica No 26 EEA INTA Mercedes, Corrientes, Argentina 84 p

    Google Scholar 

  • Hammatt N, Ghase TK, Davey MR (1986) In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 3, Academic Press, Orlando, USA, pp 67–95

    Google Scholar 

  • Mroginski LA, Kartha KK (1984) In: Janick J (ed) Plant Breeding Reviews, Vol 2, AVI Pub.Co, Westport, CT. USA, pp 215–264

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497

    Google Scholar 

  • Phillips GC, Collins GB (1984) In: Sharp WR, Evans DA, Ammirato P, Yamada Y (eds) Handbook of Plant Cell Culture, Vol 2, Macmillan Publ. Co., N.York, pp 169–210

    Google Scholar 

  • Rey HY, Bovo OA, Mroginski LA (1985) Agronomie 5:819–824

    Google Scholar 

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina

    P. N. Angeloni, H. Y. Rey & L. A. Mroginski

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  1. P. N. Angeloni
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  2. H. Y. Rey
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  3. L. A. Mroginski
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Communicated by G. C. Phillips

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Angeloni, P.N., Rey, H.Y. & Mroginski, L.A. Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum . Plant Cell Reports 11, 519–521 (1992). https://doi.org/10.1007/BF00236268

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  • DOI: https://doi.org/10.1007/BF00236268

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