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A comparison of K+ channel characteristics in human T cells: Perforated-patch versus whole-cell recording techniques

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Summary

Standard whole-cell records using the patch-clamp technique are obtained after rupturing the cell membrane just below the patch pipette. Inherent problems, such as the disruption of cellular architecture and the displacement of cytosol, are unavoidable. In the present report, a whole-cell recording technique which makes use of a monovalent cation ionophore, nystatin, was applied to lymphocytes. Nystatin-perforated patches allow electrical access to the cell interior while virtually blocking the diffusion of cellular constituents into the electrode. By comparing standard whole-cell and perforated-patch techniques we observed marked differences in: activation, inactivation, and deactivation kinetics; steady-state inactivation; and the conductance-voltage relationship of K+ currents in activated human T cells.

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Authors and Affiliations

  1. Department of Psychiatry, and Department of Anatomy and Cell Biology, Emory University, Atlanta, Georgia

    Dorothy R. Oleson, Louis J. DeFelice & Robert M. Donahoe

Authors
  1. Dorothy R. Oleson
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  2. Louis J. DeFelice
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  3. Robert M. Donahoe
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Additional information

We thank Kathleen Brattain for useful comments on the manuscript. This work was supported by NIDA grants DA04498-03 and DA01451-15, the State of Georgia Human Resources Department, and an Individual National Research Service Award presented to D.R.O. from NIAAA.

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Oleson, D.R., DeFelice, L.J. & Donahoe, R.M. A comparison of K+ channel characteristics in human T cells: Perforated-patch versus whole-cell recording techniques. J. Membarin Biol. 132, 229–241 (1993). https://doi.org/10.1007/BF00235740

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  • DOI: https://doi.org/10.1007/BF00235740

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