Summary
Morphometric analytical procedures were employed to study the pineal gland of the Mongolian gerbil following superior cervical ganglionectomy (SCGX). The purpose of this study was to define the effects of sympathetic denervation on the morphology of the gland at two time periods, 0500 and 1900 h (one hour before lights-on and lights-off, respectively). Fluorescence histochemistry was employed to determine catecholamine and indoleamine content in intact and denervated pineal glands. After SCGX, the pinealocytes decrease in size, concretions are prevented from forming, and the yellow fluorescence in the gland is lost. Following denervation a depression in the volume of most of the pinealocyte organelles, i.e., SER, RER/ribosomes, free cytoplasm, mitochondria and presumptive secretory vesicles, was also observed. However, synaptic ribbons increased in volume in the gerbils that had been killed at 1900 h. It appears that the sympathetic innervation to the pineal gland is a requirement for the presumptive secretory activity of the pinealocytes.
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Arïens Kappers, J.: The development, topographical relations and innervation of the epiphysis cerebri in the albino rat. Z. Zellforsch. 52, 163–215 (1960)
Ariëns Kappers, J.: Survey of the innervation of the epiphysis cerebri and the accessory pineal organs of vertebrates. Prog. Brain Res. 10, 87–153 (1965)
Arïens Kappers, J.: The mammalian pineal gland, a survey. Acta Neurochir. 34, 109–149 (1976)
Björklund, A., Falck, B., Lindvall, O., Svensson, L.-A.: New aspects on reaction mechanisms in the formaldehyde histofluorescence method for monoamines. J. Histochem. Cytochem. 21, 17–25 (1973)
Deguchi, T.: Tryptophan hydroxylase in pineal gland of rat: Postsynaptic localization and absence of circadian change. J. Neurochem. 28, 667–668 (1977)
Falck, B., Owman, C.: A detailed methodological description of biogenic monoamines. Acta Univ. Lund. Sec., II, 7, 1–23 (1965)
Glass, G.V., Peckham, P.D., Sanders, J.R.: Consequences of failure to meet assumptions underlying the fixed effects analyses of variance and covariance. Rev. Ed. Res. 42, 237–288 (1972)
Hori, S., Kuroda, Y., Saito, K., Ohotani, S.: Subcellular localization of tryptophan-5-mono-oxygenase in pineal glands and raphe nuclei. J. Neurochem. 27, 911–914 (1976)
Japha, J.L., Eder, T.J., Goldsmith, E.D.: Morphological and histochemical features of the gerbil pineal system. Anat. Rec. 178, 381 (1974)
Klein, D.C.: Circadian rhythms in the pineal gland. In: Endocrine Rhythms. (D.T. Krieger, ed.), pp. 203–223. New York: Raven Press (1979)
Laties, A.M., Lund, R., Jacobowitz, D.: A simplified method for the histochemical localization of cardiac catecholamine-containing nerve fibers. J. Histochem. Cytochem. 15, 535–541 (1967)
Lin, H.-S., Hwang, B.-H., Tseng, C.-Y.: Fine structural changes in the hamster pineal gland after blinding and superior cervical ganglionectomy. Cell Tissue Res. 158, 285–299 (1975)
Miline, R., Krstic, R., Devecerski, V.: Sur le comportement de la glande pinéale dans des conditions de stress. Acta Anat. (Basel) 71, 352–402 (1968)
Moore, R.Y., Heller, A., Bhatnager, R.K., Wurtman, R.J., Axelrod, J.: Central control of the pineal gland: visual pathways. Arch. Neurol. 18, 208–218 (1968)
Morgan, W.W., Reiter, RJ: Pineal noradrenaline levels in the Mongolian gerbil and in different strains of laboratory rats over a lighting regimen. Life Sci. 21, 555–558 (1977)
Nielsen, J.T., Møller, M.: Innervation of the pineal gland in the Mongolian gerbil (Meriones unguiculatus). Cell Tissue Res. 187, 235–250 (1978)
Panke, E., Rollag, M.: Pineal and plasma melatonin concentrations in the golden hamster following superior cervical ganglionectomy and pinealectomy. Anat. Rec. 190, 501 (1978)
Reiter, R.J., Sorrentino, Jr., S., Jarrow, E.L.: Central and peripheral neural pathways necessary for pineal function in the adult female rat. Neuroendocrinology 8, 321–333 (1971)
Reiter, R.J., Vaughan, M.K., Vaughan, G.M., Sorrentino, Jr., S., Donofrio, R.J.: The pineal gland as an organ of internal secretion. In: Frontiers of Pineal Physiology (M.D. Altschule, ed.), pp. 54–174. Cambridge: Harvard University Press (1975)
Reiter, R.J., Welsh, M.G., Vaughan, M.K.: Age-related changes in the intact and sympathetically denervated gerbil pineal gland. Am. J. Anat. 146, 427–432 (1976)
Romijn, H.J.: The ultrastructure of the rabbit pineal gland after sympathectomy, parasympathectomy, continuous illumination, and continuous darkness. J. Neural Transm. 36, 183–194 (1975)
Sheridan, M.N.: Pineal gland fine structure: dense-cored vesicles. In: Brain-Endocrine Interaction II. The Ventricular System. 2nd Int. Symp. (K.M. Knigge, D.E. Scott, H. Kobayashi, and S. Ishii, eds.), pp. 324–336. Basel: Karger (1975)
Sheridan, M.N., Sladek, Jr., J.R.: Histofluorescence and ultrastructural analysis of hamster and monkey pineal. Cell Tissue Res. 164, 145–152 (1975)
Vollrath, L., Huss, H.: The synaptic ribbons of the guinea-pig pineal gland under normal and experimental conditions. Z. Zellforsch. 139, 417–429 (1973)
Weibel, E.R., Bolender, R.P.: Stereological techniques for electron microscopic morphometry. In: Principles and Techniques of Electron Microscopy: Biological Applications, Vol. 3 (M.A. Hayat, ed.), pp. 237–296. New York: Van Nostrand Reinhold (1973)
Welsh, M.G., Reiter, R.J.: The pineal gland of the gerbil, Meriones unguiculatus. I. An ultrastructural study. Cell Tissue Res. 193, 323–336 (1978)
Welsh, M.G., Cameron, I.L., Reiter, R.J.: The pineal gland of the gerbil, Meriones unguiculatus. II. Morphometric analysis over a 24-hour period. Cell Tissue Res. 204, 95–109 (1979)
Wurtman, R.J., Axelrod, J., Fischer, J.E.: Melatonin synthesis in the pineal gland: effect of light mediated by the sympathetic nervous system. Science 143, 1328–1330 (1964)
Yang, H.Y.T., Neff, N.H.: Hydroxyindole-O-methyltransferase: an immunochemical study of the neuronal regulation of the pineal enzyme. Mol. Pharmacol. 12, 433–439 (1976)
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Supported by NSF grant #PCM 77-05734 to R.J.R.
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Welsh, M.G., Hansen, J.T. & Reiter, R.J. The pineal gland of the gerbil, Meriones unguiculatus . Cell Tissue Res. 204, 111–125 (1979). https://doi.org/10.1007/BF00235168
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DOI: https://doi.org/10.1007/BF00235168