Skip to main content
SpringerLink
Log in

Spontaneous somatic embryogenesis and plant regeneration from root cultures of Peucedanum palustre

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The regeneration of Peucedanum palustre (L.) Moench (milk parsley) was established for the first time via somatic embryogenesis from primary root cultures. Callus formation occurred on the root cultures and showed spontaneous embryogenic capability on B5 basal medium supplemented with a low concentration of indoleacetic acid (5.5 × 10−7 M). 2,4-Dichlorophenoxyacetic acid was not needed for the initiation of embryogenesis. The somatic embryos germinated and formed plantlets on hormone-free B5 medium. These plantlets were easily transferable to pots, and are presently passing their second growing season in the greenhouse.

Development of the somatic embryos progressed through the globular, heart-shaped, torpedo-shaped, and cotyledonary stages, typical of zygotic embryos. Synchronization performed by sieving the embryos did not affect the development time. The culture has retained its embryogenic capacity for 25 months.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

indoleacetic acid

IBA:

3-indolebutyric acid

BAP:

6-benzylaminopurine

References

  • Ammirato PV (1983) In: Evans D (ed) Handbook of plant cell culture. Vol. 1. Techniques for propagation and breeding. MacMillan Publ. Co., New York, pp. 82–123

    Google Scholar 

  • Borkird C, Choi JH, Sung ZR (1986) Plant Physiol 81:1143–1146

    Google Scholar 

  • Collin HA, Isaac S (1991) In: Bajaj YPS (eded) Bio-technology in agriculture and forestry 15. Medicinal and aromatic plants III. Springer-Verlag, pp. 73–92

  • Dudits D, Bögre L, Györgyey J (1991) J Cell Sci 99:473–483

    Google Scholar 

  • Furmanowa, M., Sowinska, D. and Pietrosiuk, A. (1991) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 15. Medicinal and aromatic plants III. Springer-Verlag, pp. 176–190

  • Gamborg O, Miller RA, Qjima K (1968) Exp Cell Res 50:151–158

    CAS  PubMed  Google Scholar 

  • Horsch RB, King J (1990) Can J Bot 58:2402–2406

    Google Scholar 

  • Jia J, Shi J, Wang Y, Zhang S (1989) Acta Bot Sin 31:361–366

    Google Scholar 

  • Mizuta S, Okuda K, Inagawa K, Tanaka I (1987) Mem Fac Sci Kochi Univ Set D (Biol) 8:29–38

    Google Scholar 

  • Nadel BL, Altman A, Ziv M. (1990) Plant Cell Tiss Org Cult 20:119–124

    Google Scholar 

  • Oksman-Caldentey K-M, Kivelä O, Hiltunen R (1991) Plant Sci 78:129–136

    Google Scholar 

  • Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, pp. 139–146, 213–229

    Google Scholar 

  • Smith SM, Street HE (1974) Ann Bot 38:223–241

    Google Scholar 

  • Vasil IK (1984) Cell culture and somatic cell genetics of plants I. Academic Press, Inc., Orlando, Florida, 825 p

    Google Scholar 

  • Vuorela H (1988) Acta Pharm Fenn 97:113–120

    CAS  Google Scholar 

  • Vuorela H, Törnquist K, Sticher O, Hiltunen R. (1988) Acta Pharm Fenn 97:167–174

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacy, Microbiology Division, University of Helsinki, P.O. Box 15, SF-00014, Finland

    Pia Vuorela

  2. Department of Pharmacy, Pharmacognosy Division, University of Helsinki, P.O. Box 15, SF-00014, Finland

    Kirsi-Marja Oksman-Caldentey, Jaana Lipponen & Raimo Hiltunen

Authors
  1. Pia Vuorela
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kirsi-Marja Oksman-Caldentey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaana Lipponen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raimo Hiltunen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Lörz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vuorela, P., Oksman-Caldentey, KM., Lipponen, J. et al. Spontaneous somatic embryogenesis and plant regeneration from root cultures of Peucedanum palustre . Plant Cell Reports 12, 453–456 (1993). https://doi.org/10.1007/BF00234711

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234711

Keywords

Search

Navigation