Abstract
Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3′ end by the soybean 7S storage protein gene. The plasmid containing the chimeric gene was delivered to the cotyledons via particle bombardment 900 PSI. Zygotic cotyledons from six soybean varieties were tested for transient expression of the β-glucuronidase gene. The level of reporter gene expression differed between genotypes. The genotypes could be classified as high, fair or poor expressors. Cotyledons from different genotypes were then bombarded at 650, 900 or 1100 PSI. GUS expression varied among genotypes independently of the pressure of bombardment. Finally, the ability of cotyledons to express the reporter gene depended on the developmental stage of the seed from which it was excised with the younger stage being the least responsive. However, genotypic specific expression remained after controlling for developmental stage of the cotyledons.
Similar content being viewed by others
Abbreviations
- ANOVA:
-
analysis of variance
- GUS:
-
β-glucuronidase
- PSI:
-
pounds per square inch
- TEUs:
-
transient expression units
- MSO3:
-
MS media with 3% sucrose
References
Bailey MA, Boerma HR, Parrott WA (1993) Crop Sci: in press
Christou P, Swain WF, Yang NS, McCabe DE (1989) Proc Nat Acad Sci USA 86: 7500–7504
Christou P, McCabe DE, Martinell BJ, Swain WF (1990) Trends in Biotech 8: 145–151
Finer JF, McMullen D (1991) In Vitro Cell Dev Biol 27: 175–182
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158
Hartweck LM, Lazzerri PA, Cui D, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 821–828
Hildebrand DF, Liu W, Deng W, Grayburn WS, Collins GB (1991) In: Rattray JBM (ed) Biotechnology and the oils and fats industry. Aberical Oil Chemistry Society Press, Champagne, IL, pp 58–75
Hinchee MA, Conner-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischoff DA, Re DB, Fraley RT, Horsch RB (1988) Bio/Tech 6: 915–922
Hood EE, Chilton WS, Chilton M-D, Fraley RT (1986) J Bacteriol 168: 1283–1290
Horsch RB, Fry JE, Hoffman NL, Eichholz D, Rogers SG, Fraley RT (1985) Science 227: 1227–1231
Hooykaas PJJ (1989) Plant Mol Biol 13: 327–336
Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405
Klein TM, Wolf ED, Wu R, Sanford JC (1987) Nature 327: 70–73
Komatsuda T (1992) Bull Natl Inst Agrobiol Resour 7: 1–78
Kudirka DT, Colburn SM, Hinchee MA, Wright MS (1986) Can J Genet Cytol 28: 808–817
Lazzeri PA, Hildebrand DF, Collins GB (1985) Plant Mol Biol Rep 3: 160–167
Li, L, Qu R, Kochko A, Fauquet C, Beachy RN (1993) Plant Cell Rep 12: 250–255
Matthysse AG, Gurlitz HG (1982) Physiol Plant Path 21: 381–387
McCabe DE, Swain WF, Martinell BJ, Cristou P (1988) Bio/Tech 6: 923–926
Moore PJ, Coffins GB (1993) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, vol 23, Springer-Verlag, Berlin Heidelberg New York, pp 228–236
Murashige T, Skoog F (1962) Physiol Plant 15: 473–494
Owens LD, Cress DE (1985) Plant Physiol 77: 87–94
Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 817–820
Parrott WA, Hoffman LM, Hildebrand DF, Williams EG, Collins GB (1989) Plant Cell Rep 7: 615–617
Parrott WA, Williams EG, Hildebrand DF, Collins GB (1989) Plant Cell, Tiss, Org Cult 16: 15–21
Pederson HC, Cristiansen J, Wyndaele R (1983) Plant Cell Rep 2: 201–204
Sato S, Newell C, Kolacz K, Tredo L, Finer J, Hinchee M (1993) Plant Cell Rep 12: 408–413
Sebastiani F, Farrell LB, Schuler MA, Beachy RN (1990) Plant Mol Biol 15: 197–201
Author information
Authors and Affiliations
Additional information
Communicated by W. A. Parrott
Rights and permissions
About this article
Cite this article
Moore, P.J., Moore, A.J. & Collins, G.B. Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons. Plant Cell Reports 13, 556–560 (1994). https://doi.org/10.1007/BF00234510
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234510