Abstract
Somatic embryogenesis was initiated from immature embryos of western larch (Larix occidentalis Nutt.) on media containing 2,4-dichlorophenoxyacetic acid and N6- benzyladenine. The effects of explant type and ammonium nitrate and glutamine concentrations on initiation were tested. Although 21–93% of explants rendered cultures in various experiments, only 3% yielded sustainable embryogenic lines. Excised embryos at the early cotyledonary stage were optimal for initiation. Maturation of somatic embryos was promoted by abscisic acid. Response to abscisic acid concentrations and duration of exposure to abscisic acid varied with genotype. Maximal results were obtained with 0.025 μ M abscisic acid for 1 to 2 weeks followed by individual culture on medium without growth regulators. Mature somatic embryos developed into shoots with roots. Plantlets have been established in peat.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
N6-benzyladenine
- IBA:
-
indole-3-butyric acid
- ABA:
-
abscisic acid
References
Ammirato PV (1974) Bot Gaz 135:328–337
Becwar MR, Noland TL, Wann SR (1987) Plant Cell Reports 6:35–38
Durzan DJ, Gupta PK (1987) Plant Sci 52:229–235
Fowells HA (1965) Silvics of forest trees of the United States. Agriculture Handbook No. 271. United States Department of Agriculture, Forest Service, Washington, pp 235–241
Hakman I, Fowke LC, von Arnold S, Eriksson T (1985) Plant Sci 38:53–59
Klimaszewska K (1989) Plant Sci 63:95–103
Litvay JD, Verma DC, Johnson MA (1985) Plant Cell Reports 4:325–328
Nagmani R, Bonga J (1985) Can J For Res 15:1088–1091
Owens JN, Molder M (1979) Can J Bot 57:2673–2690
Roberts DR, Flinn BS, Webb DT, Webster FB, Sutton BCS (1990) Physiol Plant 78:355–360
Tautorus TE, Atree SM, Fowke LC, Dunstan DI (1990) Plant Sci 67:115–124
Tautorus TE, Fowke LC, Dunstan DI (1991) Can J Bot 69:1873–1899
von Aderkas P, Klimaszewska K, Bonga J (1990) Can J For Res 20:9–14
von Arnold S (1987) J Plant Physiol 128:233–244
von Arnold S, Hakman I (1988) J Plant Physiol 132:164–169
Zeevaart JAD, Creelman RA (1988) Ann Rev Plant Physiol Plant Mol Biol 39:439–473
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Communicated by F. Constabel
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Thompson, R.G., von Aderkas, P. Somatic embryogenesis and plant regeneration from immature embryos of western larch. Plant Cell Reports 11, 379–385 (1992). https://doi.org/10.1007/BF00234365
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DOI: https://doi.org/10.1007/BF00234365