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Regeneration and Agrobacterium-mediated transformation of chrysanthemum

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Abstract

A method has been developed to regenerate shoots directly from leaf pieces of the autumn flowering chrysanthemum Dendranthema indicum (L.) Des Moul (genotype Korean). Transgenic plants of this genotype were generated using transformation mediated by the disarmed strain of Agrobacterium tumefaciens LBA4404, containing either pKIWI110 or pGA643. Both pKIWI110 and pGA643 contain the selectable marker gene neomycin phosphotransferase II (NPTII) and pKIWI110 also contains the reporter gene β-D-glucuronidase. Leaf pieces inoculated with pKIWI110 produced zones of blue cells two days after inoculation. Shoots from leaf pieces inoculated with pGA643 were selected on kanamycin. PCR and Southern analysis of shoots that were able to root on kanamycin confirmed the presence of the NPTII gene in the plant genome.

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References

  • An G, Ebert PR, Mitra A, Ha SB (1988) In: Gelvin SB, Scilperoort RA (eds) Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht, pp A3 1–19

    Google Scholar 

  • Bush SR, Earle ED, Langhans RW (1976) Amer J Bot 63: 729–737

    Google Scholar 

  • Broertjes C, Lock CAM (1985) Euphytica 34: 97–103

    Google Scholar 

  • Broertjes C, Keen A (1980) Euphytica 29: 73–87

    Google Scholar 

  • Chriqui D, David C, Adam S (1988) Plant Cell Rep 7: 11–114

    Google Scholar 

  • Deroles SC (1988) PhD Thesis, University of Auckland

  • Hoekema A, Hirsch PR, Hookyass PJJ, Schilperoort RA (1983) Nature 303: 179–180

    CAS  Google Scholar 

  • Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) Science 227: 1229–1231

    CAS  Google Scholar 

  • Hill GP (1969) Physiol Plant 21: 386–389

    Google Scholar 

  • Janssen B, Gardner RC (1990) Plant Mol Biol 14: 61–72

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405

    CAS  Google Scholar 

  • Jung-Heiliger H, Horn W (1980) Journal of Plant Breeding 85: 185–199

    Google Scholar 

  • Kaul V, Miller RM, Hutchinson JF, Richards D (1990) Plant Cell, Tissue and Organ Cult (in press)

  • Klee HJ, White FF, Iyer VN, Gordon MP, Nester EW (1983) J Bacteriol 153: 878–883

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) A laboratory manual. Cold Spring Harbor, New York

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 475–497

    Google Scholar 

Download references

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Author notes

  1. Susan E. Ledger

    Present address: Department of Cellular and Molecular Biology, University of Auckland, Private Bag, Auckland, New Zealand

Authors and Affiliations

  1. Horticultural Research Centre, Ministry of Agriculture and Fisheries, Levin, New Zealand

    Susan E. Ledger, Simon C. Deroles & Nigel K. Given

Authors
  1. Susan E. Ledger
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  2. Simon C. Deroles
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  3. Nigel K. Given
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Communicated by R. B. Horch

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Ledger, S.E., Deroles, S.C. & Given, N.K. Regeneration and Agrobacterium-mediated transformation of chrysanthemum. Plant Cell Reports 10, 195–199 (1991). https://doi.org/10.1007/BF00234294

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  • DOI: https://doi.org/10.1007/BF00234294

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