Summary
Mitochondria were isolated and purified from paired lines of safflower (Carthamus tinctorius L.) restorer and cytoplasmic male sterile plants using isopycnic gradient centrifugation in isoosmotic Percoll. Agarose gel electrophoresis of restriction endonuclease digested DNAs showed characteristic polymorphism. Restriction fragments representing about 75% of the mitochondrial genome were common to both the fertile and CMS plants, but differed significantly in stoichiometric amounts. The remaining 25% could be accounted for by unique restriction fragments observed in only one or the other plant types.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Crouzillat D, Leroy P, Perrault A, Ledoigt G: Molecular analysis of the mitochondrial genome of Helianthus annuus in relation to cytoplasmic male sterility and phylogeny. Theor Appl Genet 74: 773–780, 1987
Hanson MR, Conde MF: Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94: 213–267, 1985
Longsdale DM: Cytoplasmic male sterility: a molecular perspective. Plant Physiol Biochem 25: 265–271, 1987
Claassen CE: Inheritance of sterility, flower color, spinelessness, attached pappus and rust resistance in safflower. Carthamus tinctorius. Nebr Agric Exp Stn Bull 171, p 28, 1952
Ebert WW, Knowles PF: Inheritance of pericarp types, sterility, and dwarfness in several safflower crosses. Crop Sci 6: 579–582, 1966
Carapetian J, Rupert EA: Meiotic irregularities caused by interacting sterility genes in cultivated safflower (Carthamus tinctorius L.). Can J Genet Cytol 19: 103–109, 1977
Ebert WW: The inheritance and developmental aspects of the thin hull mutant of Carthamus tinctorius L. Dissertation Univ of Cal Davis, 1964
Heaton TC, Knowles PF: Inheritance of male sterility in safflower. Crop Sci 22: 520–522, 1982
Pay A, Smith MA: A rapid method for purification of organelles for DNA isolation: self-generated percoll gradients. Plant Cell Reports 7: 96–99, 1988
Singh BB, Rathburn H, Hedgcoth C: Isolation of mitochondrial DNA from cytoplasmic male sterile and maintainer lines of pearl millet Pennisetum americanum (L.) Leeke. Theor Appl Genet 74: 168–170, 1987
Bland MM, Matzinger DF, Levings CS: Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species. Theor Appl Genet 69: 535–541, 1985
Ma C, Smith MA: Physical and genetic mapping of safflower chloroplast DNA. Plant Mol Biol 4: 385–388, 1987
Kolodner R, Tewarii KK: The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta 402: 372–390, 1975
Asahi T, Kumashiro T, Kubo T: Constitution of mitochondrial and chloroplast genomes in male sterile tobacco obtained by protoplast fusion of Nicotiana tabacum and N. debneyi. Plant Cell Physiol 29: 43–49, 1988
Boutin V, Pannenbecker G, Ecke W, Schewe G, SaumitouLaprade P, Jean R, Vernet Ph, Michaelis G: Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet 73: 625–629, 1987
Borck KS, Walbot V: Comparison of the restriction endonuclease digestion patterns of mitochondrial DNA from normal and male sterile cytoplasms of Zea mays L. Genetics 102: 109–128, 1982
Small ID, Isaac PG, Leaver CJ: Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J 6: 865–869, 1987
Palmer JD: Comparative organization of chloroplast genomes. Ann Rev Genet 19: 325–354, 1985
Pring DR, Gengenbach BG, Wise RP: Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum. Phil Trans R Soc Lond B319: 187–198, 1988
Mackenzie SA, Pring DR, Bassett MJ, Chase CD: Mitochondrial DNA rearrangements associated with fertility restoration and cytoplasmic reversion to fertility in cyto plasmic male sterile Phaseolus vulgaris L. Proc Nat Acad Sci USA 85: 2714–2717, 1988
Pring DR, Lonsdale DM: Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97: 1–46, 1985
Markaroff CA, Palmer JD: Mitochondrial DNA Rearrangements and transcriptional alterations in the male-sterile cytoplasm of ogura radish. Mol Cell Biol 8: 1474–1480, 1988
Kadowaki K, Osumi T, Nemoto H, Harada K, Shinjyo C: Mitochondrial DNA polymorphism in male-sterile cytoplasm of rice. Theor Appl Genet 75: 234–236, 1988
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pay, A., Smith, M.A. The mitochondrial genome of safflower: Isolation and restriction fragment analysis of DNA from CMS and restorer lines. Mol Cell Biochem 100, 151–157 (1991). https://doi.org/10.1007/BF00234164
Issue Date:
DOI: https://doi.org/10.1007/BF00234164