Abstract
Cell suspension cultures, which retained embryogenic potential for almost 2 years, were established from young, expanding, juvenile leaves of a mature Thevetia peruviana L. tree. Calli were obtained by culturing young leaf discs on MS medium supplemented with 2 mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.1 mg/L kinetin. Suspension cultures were initiated by transfer of calli to liquid medium containing 1 mg/L 2,4-D + 0.1 mg/L kinetin, and the cultures were maintained by subculturing to fresh medium at 2 week intervals. Embryogenic frequency of cell aggregates was more than 80% when plated on semi-solid medium containing 0.1 mg/L 2, 4-D and 2 mg/L 2-isopentenyladenine (2-iP). Cell aggregates with developing embryos were transferred to fresh medium lacking growth regulators for embryo maturation. Early embryo development was synchronous and a large number of somatic embryos were produced. These somatic embryos developed into plantlets upon subsequent transfer to modified half-strength MS medium. More than 200 green and rooted plants, at an average of 80 plants per 100 mg of embryogenic callus, were obtained with 60% survival under glass house conditions.
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Abbreviations
- 2, 4-D:
-
2 4-dichlorophenoxyacetic acid
- 2-iP:
-
2-isopentenyladenine
- IAA:
-
Indole — 3 — acetic acid
- KN:
-
Kinetin
- MS:
-
Murashige and Skoog (1962) basal medium
- NAA:
-
1 -Napthalene acetic acid
References
Baker CM, Wetzstein HY (1992) Plant Cell Reports 11: 71–75
Bhakuni DS (1990). Sci Rep 8: 12–17
Carman JG (1990) In Vitro Cell Dev Biol 26: 746–753
Datta K, Datta SK (1985) Curr Sci 54: 248–250
Eapen S, George L (1989) Plant Sci 61: 127–130
Finer JJ (1988) Plant Cell Reports 7: 399–402
Gupta PK, Durjan DJ (1987) Bio/Technology 5: 147–151
Guimaraes MLS, Cruz GS, Montezuma-de-Carvalho JM (1988) Plant Cell Tiss Org Cult 15: 161–167
Haccius B (1978) Phytomorphology 28: 74–81
Hatanaka T, Arakawa O, Yasuda T, Uchida N, Yamaguchi T (1991) Plant Cell Reports 10: 179–182
Kumar A (1992a) Ind J Exp Biol 30: 749–750
Kumar A (1992b) plant Cell Tiss Org Cult 31: 47–50
Mariotti D, Arcioni S (1983) Plant Cell Tiss Org Cult 2: 103–110
Meijer EG, Brown DC (1987) Physiol Plant 69: 591–596
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nagmani R, Beewar MR, Wann SR (1987) Plant Cell Reports 6: 157–159
Raghav Ram NV, Nabors MW (1984) Z Pflanzenphysiol 113: 315–323
Rhodes CA, Lowe KS, Ruby KL (1988) Bio/Technology 6: 56–60
Trolinder NL, Godwin JR (1987) Plant Cell Reports 6: 178–181
Tremonilloux-Guiller J, Chenieux JC (1991) Plant Cell Reports 10: 102–105
Umbeck P, Johnson G, Barton K, Swain W (1987) Bio/Technology 5: 263–266
von Arnold S (1988) News Letter IAPTC 56: 2–13
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Communicated by G. C. Phillips
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Sharma, A., Kumar, A. Somatic embryogenesis and plant regeneration from leaf-derived cell suspension of a mature tree — Thevetia peruviana L.. Plant Cell Reports 14, 171–174 (1994). https://doi.org/10.1007/BF00233784
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DOI: https://doi.org/10.1007/BF00233784