Summary
Anti-mitochondrial autoantibody and fluorescent derivatives of insulin stain phase-dense mitochondria in acetone-fixed monolayers of fibroblasts. Double fluorochrome studies show mitochondria in close topographic association with intermediate filaments. In cells treated with vinblastine or colchicine, mitochondria are relocated in sites closely associated with coils of perinuclear intermediate filaments. In contrast, autoantibody to polyribosomes stains granules aligned in the long axis of well spread embryonic cells, in the direction of actin-containing fibrils, an arrangement that is lost in cells pretreated with the actin filament disrupting drug cytochalasin B. In more mature fibroblasts, antiribosomal antibody reacts with phase-dense rough endoplasmic reticulum and this staining pattern is not affected by cytochalasin B. The observations suggest that mitochondria are associated with intermediate filaments and that free polyribosomes, but not polyribosomes attached to rough endoplasmic reticulum, are associated with cytoplasmic actin.
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References
Bray D (1979) Cytochalasin action. Nature 282:671
Bryan J (1972) Vinblastine and microtubules. II. Characterization of two protein subunits from the isolated crystals. J Mol Biol 66:157–168
Doniach D, Roitt IM, Walker JG, Sherlock S (1966) Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications. Clin Exp Immunol 1:237–262
Heggeness M, Simon M, Singer SJ (1968) Association of mitochondria with microtubules in cultured cells. Proc Natl Acad Sci 75:3863–3866
Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–256
Lazarides E, Weber K (1974) Actin antibody: the specific visualization of actin-containing filaments in non-muscle cells. Proc Natl Acad Sci 71:2268
Lee CS, Morgan G, Wooding FBP (1979) Mitochondria and mitochondria-tonofilament-desmosomal associations in the mammary gland secretory epithelium of lactating cows. J Cell Sci 38:125–135
Lenk R, Ransom L, Kaufmann Y, Penman S (1977) A cytoskeletal structure with associated polyribosomes obtained from Hela cells. Cell 10:67–78
Lolait SJ, Toh BH (1980) Binding sites of fluorescent derivatives of insulin in nuclei, rough endoplasmic reticulum and mitochondria. Cell Tissue Res (in press)
Mathy J-P, Baum R, Toh BH (1980) Autoantibody to ribosomes and systemic lupus erythematosus. Clin Exp Immunol (in press)
Nairn RC (1976) Fluorescent Protein Tracing. 4th Ed, Churchill-Livingstone, Edinburgh
Rose GP, Pomerat CM (1960). Phase contrast observations of the endoplasmic reticulum in living tissue cultures. J Biophys Biochem Cytol 8:423–430
Starger JM, Goldman RD (1977) Isolation and preliminary chracterization of 10mm filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci 74:2422–2426
Toh BH, Hard GC (1977). Actin co-caps with concanavalin A receptors. Nature 269:695–697
Toh BH, Gallichio HA, Jeffrey PL, Livett BG, Muller HK, Cauchi NM, Clarke FM (1976). Anti-actin stains synapses. Nature 264:648–650
Toh BH, Yildiz A, Sotelo J, Osung O, Holborow EJ, Kanakoudi F, Small JV (1979). Autoantibodies to intermediate filaments and viral infections. Clin Exp Immunol 37:76–82
Wessels NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wrenn JT, Yamada KM (1971) Microfilaments in cellular and developmental processes. Science 171:135–143
Yildiz A, Toh BH, Sotelo J, Osung O, Holborow EJ, Small JV (1980). Smooth muscle autoantibodies reacting with cytoplasmic intermediate filaments in sera from normal nonimmunized rabbits. Clin Immunol Immunopathol (in press).
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Supported by a grant from the Anti-Cancer Council of Victoria. We thank Mrs. I. Burns for technical assistance and Dr. H.A. Ward and staff for preparation of fluorescent conjugates
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Toh, B.H., Lolait, S.J., Mathy, J.P. et al. Association of mitochondria with intermediate filaments and of polyribosomes with cytoplasmic actin. Cell Tissue Res. 211, 163–169 (1980). https://doi.org/10.1007/BF00233731
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DOI: https://doi.org/10.1007/BF00233731