Summary
The preputial glands of male nude (nu/nu) mice were analyzed by a combination of electron microscopy and gas chromatography to determine whether or not they are affected, like developing hairs and nails, by the nu/nu genotype. Results of the analyses revealed no differences between the glands of nude and normal male mice in either their ultrastructural characteristics or lipid secretory products.
Similar content being viewed by others
References
Beaver DL (1963) Electron microscopy of the acinar cell of the preputial gland. Anat Rec 146:47–59
Birch MC (ed) (1974) Pheromones. North-Holland Pub Co, Amsterdam
Bronson FH (1966) A sex attractant function for mouse preputial gland. Bull Ecol Soc Am 47:182 (Abstr)
Bronson FH (1971) Rodent pheromones. Biol Reprod 4:344–357
Bronson FH (1974) Pheromonal influences on reproductive activities in rodents. In: Birch MC (ed) Pheromones. North-Holland Pub Co, Amsterdam, pp 344–365
Bronson FH, Caroom D (1971) Preputial gland of the male mouse; attractant function. J Reprod Fertil 25:279–282
Brown JC, Williams JD (1972) A histochemical study of the preputial glands of the male laboratory rat and mouse. Acta Anat 81:270–285
Bruce HM (1970) Pheromones. Brit Med Bull 26:10–13
Chipman RK, Albrecht ED (1974) The relationship of the male preputial gland to the acceleration of oestrus in the laboratory mouse. J Reprod Fertil 38:91–96
Cunha GR (1975) Hormonal influences on the morphogenesis of the preputial gland of embryonic mice. Anat Rec 181:35–54
Dangelo JC, Munger BL (1964) The ultrastructure of the rat preputial gland. J Ultrastruct Res 11:230–245
Deitch AD (1966) Cytophotometry of nucleic acids. In: Wied GL (ed) Introduction to Quantitative Cytochemistry. Academic Press, New York, pp 327–354
Ellis JF Jr, Ikenberry RD (1978) Pheromonal gland studies of selected rodents. J Tenn Acad Sci 54:73 (Abstr)
Fawcett DW, Long JA, Jones AL (1969) The ultrastructure of endocrine glands. Rec Progr Horm Res 25:315–380
Flanagan SP (1966) “Nude”, a new hairless gene with pleiotropic effects in the mouse. Genet Res Camb 8:295–309
Gilula NB (1977) Gap junctions and cell contacts. In: Karkinen-Jääskeläinen M, Saxén L, Weiss L (eds) Cell Interactions in Differentiation. Academic Press, London, pp 325–338
Green EL (1966) Biology of the Laboratory Mouse. McGraw-Hill, New York
Ito S, Winchester RJ (1963) The fine structure of the gastric mucosa in the bat. J Cell Biol 16:541–577
Jones RB, Nowell NW (1973) Effects of preputial and coagulating gland secretions upon aggressive behaviour in male mice: a confirmation. J Endocrinol 59:203–204
Kitson N, Van Lennep EW, Young JA (1978) Gap junctions in human sebaceous glands. Cell Tissue Res 190:115–121
Lillie RD, Fullmer HM (1976) Histopathologic Technic and Practical Histochemistry. McGraw-Hill, New York, 4th ed
McKinney TD, Christian JJ (1970) Effect of preputialectomy on fighting behavior in mice. Proc Soc. Exp Biol Med 134:291–293
Montagna W (1963) Comparative aspects of sebaceous glands: In: Montagna W, Ellis RA, Silver AF (eds) Advances in Biology of Skin. Macmillan, New York, Vol 4, pp 32–45
Mugford RA, Nowell NW (1970) The aggression of male mice against androgenized females. Psychonom Sci 20:191–192
Mugford RA, Nowell NW (1971) The preputial glands as a source of aggression-promoting odors in mice. Physiol Behav 6:247–249
Ozawa H (1967) The fine structure of preputial glands in mice. Acta Anat Nippon 42:181–208
Percy JE, Weatherston J (1974) Gland structure and pheromone production in insects. In: Birch MC (ed.) Pheromones. North-Holland Pub Co, Amsterdam, pp 11–34
Prutkin L, Rossman TG, Wheatley VR (1977) Ultrastructural changes and viral morphogenesis during the growth of the mouse preputial gland tumor ESR 586. Cancer Res 37:551–556
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Rietschel PE (1929) Zur Morphologie und Histologie der Genitalausführungsgänge im Individualcyclus der weißen Maus. Z Wissenschaft Zool 135:428–494
Rudali G, Roudier R, Vives Cl (1974) The preputial gland of the male mouse. Pathol Biol 22:895–899
Sansone G, Swartzendruber DC, Snyder F (1970) Inclusions in mitochondria of preputial glands from mice. A combined biochemical and morphologic study. Biochim Biophys Acta 201:401–409
Schaffer J (1933) Die Vorhautdrüsen von Maus und Ratte. Z Zellforsch 34:1–22
Sippel TO (1973) The histochemistry of thiols and disulphides. I. The use of N-(4-aminophenyl) maleimide for demonstrating thiol groups. Histochem J 5:413–423
Sippel TO: (1978a) The histochemistry of thiols and disulphides. II. Methodology of differential staining. Histochem J 10:585–595
Sippel TO (1978b) The histochemistry of thiols and disulphides. III. Staining patterns in rat tissues. Histochem J 10:597–609
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Strauss JS, Matoltsy AG (1977) Skin, Chapter 16. In: Weiss L, Greep RO (eds) Histology. McGraw-Hill, New York, 4th ed, pp 575–613
Swift H (1966) The quantitative cytochemistry of RNA. In: Wied GL (ed) Introduction to Quantitative Cytochemistry. Academic Press, New York, pp 355–386
Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478
Wattebled S, Bitsch J Rousset A (1978) Ultrastructure of pheromone-producing eversible vesicles in males of Chrysopa perla L. (Insecta, Neuroptera). Cell Tissue Res 194:481–496
Welsh C, Ikenberry RD (1980) Periodicity in release of a pheromone produced by the preputial glands of laboratory mice. J Tenn Acad Sci 55:55 (Abstr.)
Whitten WK (1966) Pheromones and mammalian reproduction. In: McLaren A (ed) Adavances in Reproductive Physiology, Academic Press. Vol 1, pp 155–177
Willey RL (1971) Microtechniques. A Laboratory Guide. Macmillan, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ikenberry, R.D., Curtis, S.K. & Cowden, R.R. Ultrastructural and gas-chromatographic analysis of the preputial glands of male nude (nu/nu) mice. Cell Tissue Res. 211, 73–82 (1980). https://doi.org/10.1007/BF00233724
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00233724