Summary
Investigations were performed on the influence of the phospholipid composition and physicochemical properties of the rat liver microsomal membranes on acyl-CoA synthetase and acyl-CoA : 1-acyl-sn-glycero-3-phosphocholine O-acyltransferase activities. The phospholipid composition of the membranes was modified by incubation with different phospholipids in the presence of lipid transfer proteins or by partial delipidation with exogenous phospholipase C and subsequent enrichment with phospholipids. The results indicated that the incorporation of phosphatidylglycerol, phosphatidylserine and phosphatidylethanolamine induced a marked activation of acyl-CoA synthetase for both substrates used—palmitic and oleic acids. Sphingomyelin occurred as specific inhibitor for this activity especially for palmitic acid. Palmitoyl-CoA: and oleoyl-CoA : lacyl-sn-glycero-3-phosphocholine acyltransferase activities were found to depend on the physical state of the membrane lipids. The alterations in the membrane physical state were estimated using two different fluorescent probes—1,6-diphenyl-1,3,5-hexatriene and pyrene. In all cases of membrane fluidization this activity was elevated. On the contrary, in more rigid membranes obtained by incorporation of sphingomyelin and dipalmitoylphosphatidylcholine, acyltransferase activity was reduced for both palmitoyl-CoA and oleoyl-CoA. We suggest a certain similarity in the way of regulation of membrane-bound acyltransferase and phospholipase A2 which both participate in the deacylation-reacylation cycle.
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References
Bar-Tana, J., Rose, G., Shapiro, B. 1971. The purification and properties of microsomal palmitoyl-Coenzyme A synthetase. Biochem. J. 122:353–362
Batenburg, J.J., Longmore, W.J., Klazinga, W., Van Golde, L.M.G. 1979. Lysolechitin acyltransferase and lysolecithin: lysolechitin acyltransferase in adult rat lung alveolar type II epithelial cells. Biochim. Biophys. Acta 573:136–144
Colard, O., Bard, D., Bereziat, G., Polonovski, J. 1980. Acylation of endogenous phospholipids and added lysoderivatives by rat liver plasma membranes. Biochim. Biophys. Acta 618:88–97
Davidson, B.C., Cantrill, R.C. 1986. Rat hepatocyte plasma membrane acyl-CoA synthetase activity. Lipids 21:571–574
Dix, J.A., Verkman, A.S. 1990. Pyrene eximer mapping in cultured fibroblasts by ratio imaging and time-resolved microscopy. Biochemistry 29:1949–1953
Folch, J., Lees, M., Sloane-Stanley, G.H. 1957. A simple method for isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497–507
Galla, H.-J., Hartmann, W. 1980. Eximer-forming lipids in membrane research. Chem. Phys. Lipids 27:199–219
Galla, H.-J., Sackmann, E. 1974. Lateral diffusion in the hydrophobic region of membranes. Use of pyrene excimers as optical probes. Biochim. Biophys. Acta 339:103–115
Garland, P.B., Yates, D.W., Haddock, B.A. 1970. Spectrophotometric studies of acyl-Coenzyme A synthetase of rat liver mitochondria. Biochem. J. 119:553–564
Hasegawa-Sasaki, H., Ohno, K. 1975. Acyltransferase activities in rat lung microsomes. Biochim. Biophys. Acta 380:486–495
Hill, E.E., Lands, W.E.M. 1970. Phospholipid methabolism. In: Lipid Metabolism. S.J. Wakil, editor, pp. 185–227. Academic, New York
Iritani, N., Ikeda, Y., Kajitani, H. 1984. Selectivities of 1-acylglycerophosphorylcholine acyltransferase and acyl-CoA synthetase for n — 3 polyunsaturated fatty acids in platelets and liver microsomes. Biochim. Biophys. Acta 793:416–422
Kahovkova, J., Odavic, R. 1969. A simple method for analysis of phospholipids separated on thin-layer chromatography. J. Chromatogr. 40:90–95
Kornberg, A., Pricer, W.E. 1953. Enzymatic synthesis of the coenzyme A. J. Biol. Chem. 204:329–343
Lands, E.M., Inoue, M., Sugiura, Y., Okuyama, H. 1982. Selective incorporation of polyunsaturated fatty acids into phosphatidylcholine by rat liver microsomes. J. Biol. Chem. 257:14968–14972
Lands, W.E.M., Hart, P. 1965. Specificities of acyl coenzyme A: phospholipid acyltransferases. J. Biol. Chem. 240:1905–1911
Lowry, R.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275
Marcel, Y.L., Suzue, G. 1972. Kinetic studies on the specificity of long chain acyl coenzyme A synthetase from rat liver microsomes. J. Biol. Chem. 247:4433–4436
Miyazawa, S., Hashimoto, T., Yokota, S. 1985. Identity of longchain acyl-coenzyme A synthetase of microsomes, mitochondria, and peroxisomes in rat liver. J. Biochem. 98:723–733
Momchilova, A.B., Petkova, D.H., Koumanov, K.S. 1986. Phospholipid composition modifications influence phospholipase A2 activity in rat liver plasma membranes. Int. J. Biochem. 18:945–952
Momchilova-Pankova, A.B., Markovska, T.T., Koumanov, K.K. 1990. Acyl-CoA: 1-acyl-glycero-3-phosphoethanolamine O-acyltransferase and liver plasma membrane fluidity. Biochimie. 72:863–866
Norum, K.R., Farstad, M., Bremer, J. 1966. The submitochondrial distribution of acid: CoA ligase (AMP) and palmitylCoA : carnitine palmityltransferase in rat liver mitochondria. Biochem. Biophys. Res. Commun. 24:797–804
Okuyama, H., Lands, W.E.M. 1972. Variable selectivities of acyl coenzyme A: monoacylglycereophosphate acyltransferases in rat liver. J. Biol. Chem. 247:1414–1423
Pande, S.V., Mead, J.F. 1968. Long chain fatty acids activation in subcellular preparations from rat liver. J: Biol. Chem. 243:352–361
Petkova, D.H., Momchilova-Pankova, A.B., Koumanov, K.S. 1987. Effect of liver plasma membrane fluidity on endogenous phospholipase A2 activity. Biochimie 69:1251–1255
Pugh, E.L., Kates, M. 1984. The dietary regulation of acyltransferase and desaturase activity in microsomal membranes of rat liver. Lipids 19:48–55
Sanford, G.L., Fronsolono, M.F. 1983. The role of acyl transferase in the biosynthesis of pulmonary microsomal phosphatidylglycerol. Biochem. Biophys. Res. Commun. 116:23–29
Shindo, Y., Hashimoto, Y. 1978. Acyl-coenzyme A synthetase and fatty acid oxidation in rat liver peroxisomes. J. Biochem. (Tokyo) 84:1177–1187
Shinitzky, M., Barenholz, Y. 1974. Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate. Biochemistry 249:2652–2657
Singh, H., Poulos, A. 1988. Distinct long chain and very long chain fatty acyl CoA synthetases in rat liver peroxisomes and microsomes. Arch. Biochem. Biophys. 266:486–495
Stahl, W.L., Trams, E.G. 1968. Synthesis of lipids by liver plasma membranes. Incorporation of acyl-coenzyme A derivatives into membrane lipids in vitro. Biochim. Biophys. Acta 163:459–471
Stein, Y., Widnell, C., Stein, O. 1968. Acylation of lysophospholipids by plasma membrane fractions of rat liver. J. Cell. Biol. 39:185–192
Touchstone, J.C., Chen, J.C., Beaver, K.M. 1980. Improved separation of phospholipids in thin-layer chromatography. Lipids 15:61–62
Van Blitterswijk, M.J., Van Hoeven, R.P., Van der Meer, B.W. 1981. Lipid structure order parameter (reciprocal of fluidity) in biomembranes derived from steady-state fluorescence polarization measurement. Biochim. Biophys. Acta 664:322–333
Van Heusden, P.G.H., Vianen, G.M., Van den Bosch, H. 1980. Differentiation between acyl coenzyme A: Lysophosphatidylcholine: lysophosphatidylcholine transacylase in the synthesis of dipalmitoylphosphatidylcholine in rat lung. J. Biol. Chem. 255:9312–9318
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Koshlukova, S.E., Momchilova-Pankova, A.B., Markovska, T.T. et al. Phospholipid dependence of rat liver microsomal acyl: CoA synthetase and acyl-CoA : 1-Acyl-sn-glycero-3-phosphocholine O-acyltransferase. J. Membarin Biol. 127, 113–119 (1992). https://doi.org/10.1007/BF00233283
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DOI: https://doi.org/10.1007/BF00233283