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Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)

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Abstract

Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0°C/min). A cooling rate of 0.5°C/min provided a satisfactory recovery rate (30%) from cryopreserved embryogenic callus cultures and was superior to a cooling rate of 1°C/min. Regenerated plants from cell suspension and embryogenic callus cultures cryopreserved for more than four years exhibited normal morphology, growth and boll set upon transfer to soil.

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Abbreviations

DMSO:

dimethylsulfoxide

MS:

Murashige and Skoog (1962)

MMS:

modified MS

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. J. G. Boswell Company, 850 Plymouth Avenue, P.O. Box 787, 93212-0787, Corcoran, CA, USA

    Kanniah Rajasekaran

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  1. Kanniah Rajasekaran
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Communicated by I. K. Vasil

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Rajasekaran, K. Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.). Plant Cell Reports 15, 859–864 (1996). https://doi.org/10.1007/BF00233157

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  • DOI: https://doi.org/10.1007/BF00233157

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