Abstract
The effect of benzyl viologen (a stimulator of free radical production in cells) on lipid composition, fluidity and enzymes involved in both polyunsaturated fatty acid biosynthesis and cholesterol metabolism was studied in liver microsomal membrane of adult rats. In viologen-treated animals, a significant decrease in the levels of free cholesterol and cholesteryl esters, accompanied to a decrease at the free cholesterol/phospholipid ratio, were observed. The levels of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and acyl-coenzyme A : cholesterol acyltransferase (ACAT) were also lower in viologen-treated rats than in controls. Linoleic and arachidonic acids were both severely lower while docosatetraenoic, docosapentaenoic and docosahexaenoic acids were significantly higher as compared with controls. Furthermore, a decrease in monounsaturated/saturated ratio was found. In addition, the treatment evoked a depression in the fatty acid desaturation complex, with a diminish of Δ9, Δ9, and Δ5 desaturase activities in microsomal membrane.
It was concluded that changes in phospholipid microsomal fatty acid and cholesterol content could be directly responsible for changes in membrane fluidity and function, and that extensive yield of docosahexaenoic acid may serve to maintain the physical characteristics of particular domains against oxidative stress caused by benzyl viologen treatment.
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Bhuyan KC, Bhuyan DK: Molecular mechanism of caractogenesis: III. Toxic metabolites of oxygen as initiators of lipid peroxidation and cataract. Curr Eye Res 3: 67–81, 1984
Rabinowitch HD, Fridovich I: Superoxide radicals, superoxide dismutases and oxygen toxicity in plants. Photochem Photobiol 37: 679–690, 1983
Sandy MS, Moldeus P, Ross D, Smith MT: Role of redox cycling and lipid peroxidation in bypyridyl herbicide cytotoxicity. Studies with a compromised isolated hepatocyte model system. Biochem Pharmacol 35: 3095–3101, 1986
Kappus H: Oxidative stress in chemical toxicity. Arch Toxicol 60: 144–149, 1987
Ruch RJ, Klaunig JE: Inhibition of mouse hepatocyte intercellular communication by paraquat-generated oxygen free radicals. Toxicol Appl Pharmacol 94: 427–436, 1988
Borg DC, Schaich KM, Elmore JJ, Bell JA: Cytotoxic reactions of free radical species of oxygen. Photochem Photobiol 28: 887–907, 1978
Loeper J, Emerit J, Goy J, Bedu O, Loeper J: Lipid peroxidation during human atherosclerosis. IRCS Med Sci 11: 1034–1035,1983
Ross JH, Krieger RI: Toxicity of 1,1′-alkyl-4,4′-bipyridylium salts in the rat. Drug Chem Toxicol 2: 207–222, 1979
Singh A: Chemical and biochemical aspects of superoxide radicals and related species of activated oxygen. Can J Physiol Pharmacol 60: 1330–1345, 1982
Greenberg SR, Eisenberg GM: The pulmonary effects of benzyl viologen in Swiss mice. Environ Pollut 12: 255, 1977
Muriana FJG, Santamaría C, Ruiz-Gutierrez V: Ultrastructural alteration in the rat liver produced by benzyl viologen. Exp Pathol 32: 65–72, 1987
Field FJ, Albright EJ, Mathur SN: Effect of dietary n-3 fatty acids on HMG-CoA reductase and ACAT activities in liver and intestine of the rabbit. J Lipid Res 28: 50–58,1987
Brenner RR: Effect of unsaturated acids on membrane structure and enzyme kinetics. Prog Lipid Res 23: 69–96, 1984
Periago JL, Pita ML, Sanchez del Castillo MA, Caamaño G, Suarez MD: Changes in lipid composition of liver microsomes and fatty acyl-CoA desaturase activities induced by medium chain triglyceride feeding. Lipids 24: 383–388,1989
Shapiro DJ, Nordstrom JL, Mitschelen JJ, Rodwell VW, Schimke RT: Micro assay for 3-hydroxy-3-methyl-glutaryl CoA reductase in rat liver and in L-cells fibroblasts. Biochim Biophys Acta 370: 369–377, 1974
Molina MT, Vazquez CM, Ruiz-Gutierrez V: Changes in both acyl-CoA: cholesterol acyltransferase activity and microsomal lipid composition in rat liver induced by distalsmall-bowel resection. Biochem J 260: 115–119, 1989
Brasitus Ta, Dudeja PK: Alterations in the physical state and composition of brush border membrane lipids of rat enterocytes during differentiation. Arch Biochem Biophys 240:483–488,1985
Shinitzky M, Barenholz Y: Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate. J Biol Chem 249: 2652–2657, 1974
Van Blitterswijk W, Van Hoeven RP, Van Der Meer BW: Lipid structural order parameters (reciprocal of fluidity) in biomembranes derived from steady-state fluorescence polarization measurements. Biochim Biophys Acta 644: 323–332,1981
Folch J, Lees M, Sloane-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957
Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC: Enzymic determination of total serum cholesterol. Clin Chem 20: 470–475, 1974
Bartlett GR: Phosphorous assay in column chromatography. J Biol Chem 234: 466–470, 1959
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–272,1951
Innis SM: The activity of 3-hydroxy-methylglutaryl-CoA reductase and acyl-CoA: cholesterol acyltransferase in hepatic microsomes from males, females and pregnant rats. The effect of cholestyramine treatment and the relationship of the enzyme activity of microsomal lipid composition. Biochim Biophys Acta 875: 355–361, 1986
Spector AA, Kaduce TL, Dane RW: Effect of dietary fat saturation on acyl CoA:cholesterol acyltransferase activity of rat liver microsomes. J Lipid Res 21: 169–179, 1980
Mathur SN, Armstrong ML, Alber CA, Spector AA: Hepatic acyl CoA:cholesterol acyltransferase activity during diet-induced hypercholesterolemia in cynomolgus monkeys. J Lipid Res 22: 659–667, 1981
Jeffcoat R, James AT: The regulation of desaturation and elongation of fatty acids in mammals. In: S. Numa (ed.) Fatty Acid Metabolism and its Regulation. Elsevier Science, Amsterdam, 1984, pp 85–112
Sprecher H: The influence of dietary alterations, fasting and competitive interactions on the microsomal chain elongation of fatty acids. Biochim Biophys Acta 360: 113–123, 1974
Gage JC: The action of paraquat and diquat on the respiration of liver cell fractions. Biochem J 109: 757–761, 1968
Christon R, Fernandez Y, Cambon-Gros C, Periquet A, Deltour P, Leger C, Mitjavila S: The effect of dietary essential fatty acid deficiency on the composition and properties of the liver microsomal membrane of rats. J Nutr 118: 1311–1318,1988
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Muriana, F.J.G., Ruiz-Gutierrez, V. & Vázquez, C.M. Effect of benzyl viologen on the phospholipid fatty acid composition and some properties in hepatic microsomal membrane of rats. Mol Cell Biochem 108, 125–131 (1991). https://doi.org/10.1007/BF00233116
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DOI: https://doi.org/10.1007/BF00233116