Summary
The removal of calcium ions from Murashige and Skoog culture medium induced a marked increase in the accumulation of cardenolides in cell suspension cultures of Digitalis thapsi. Cell viability was not affected although growth was slightly reduced. Strontium ions could substitute for calcium in inhibiting cardenolide production, this effect of calcium being reversed by the addition of LaCl3 or ethyleneglycol-bis-(β-aminoethyl ether)-N,N′-tetraacetic acid. The results suggest that calcium, apart from its general effects on growth, may play a role in the regulation of cardenolide metabolism in a concentration dependent manner.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzylaminopurine
- 2 4-D:
-
2,4-dichlorophenoxyacetic acid
- EGTA:
-
ethyleneglycol-bis-(β-aminoethyl ether)-N,N′-tetraacetic acid
- FW:
-
fresh weight
- MS:
-
Murashige and Skoog (1962)
References
Brisa MC, Pérez-Bermúdez P, Falcó JM, Segura J (1991) J Plant Physiol 137: 635–637
Corchete MP, Sánchez JM, Cacho M, Morán M, Fernádez-Tárrago J (1990) J Plant Physiol 137: 196–200
Corchete MP, Jiménez MA, Morán M, Cacho M, Fernández-Tárrago J (1991) Plan Cell Reports 10: 394–396
Gilroy S, Hughes WA, Trewavas AJ (1986) FEBS Lett 199: 217–221
Hepler PK, Wayne RO (1985) Ann Rev Plant Physiol 36: 397–439
Hagimori M, Matsumoto T, Obi Y (1983) Agric Biol Chem 47: 565–571
Indrayanto G, Rahayu L, Rahman A, Noeraeni PE (1993) Planta Med 59: 97–98
Kauss H (1987) Ann Rev Plant Physiol 38: 47–72
Luckner M, Diettrich B (1985) In: Neumann KH, Barz W and Reinhard E (eds.) Primary and secondary metabolism of plant cultures. Spring-Verlag, Berlin Heidelberg New York Tokyo, pp 154–163
Luckner M, Diettrich B (1991) In: Nrjkamp HJJ, Van der Plas LHW, Van Aartrijk J (eds.) Progress in Plant Cellular and molecular Biology. Kluwer Academic Publishers, Dordrecht Boston London, pp 744–753
Murashige T, Skoog F (1962) Physiol Plantarum 15: 473–497
Sierra MI, Dagnino D, Van der Heijden R, Verpoorte R. (1991) In: Lobarzewski J, Greppin H, Penel C, Gaspar Th (eds.) Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. University of Geneva, pp 295–304
Schübel H, Ruyter CM, Stöckigt J (1989) Phytochemistry 28: 491–494
Tester M (1990) New Phytol 114: 305–340
Widhohn JM (1972) Stain technology 47: 189–194
Zook MN, Rush JS, Kuc JA (1987) Plant Physiol 84: 520–525
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Cacho, M., Morán, M., Tárrago, J.F. et al. Calcium restriction induces cardenolide accumulation in cell suspension cultures of Digitalis thapsi L.. Plant Cell Reports 14, 786–789 (1995). https://doi.org/10.1007/BF00232923
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232923