Abstract
Plantlets were regenerated from 5-year subcultured compact callus derived from petiole tissues of wild viola (Viola patrinii DC.) but not from 5-year subcultured friable callus. Regeneration occurred most efficiently on medium that contained two-fold diluted basal salts of Murashige and Skoog's (MS) medium, 5 × 10−6 M 1-naphthaleneacetic acid and 10−6 M kinetin. The effect of dilution of MS basal salts could also be achieved solely by two-fold dilution of the potassium dihydrogen phosphate in the mixture.
The present study revealed that dilution of MS basal salts, in particular of potassium dihydrogen phosphate, was important for the regeneration of wild viola. Moreover, although the callus had been subcultured for 5 years, regeneration of plantlets from callus was still possible. In addition, scanning electron microscopy revealed that details of the process of plant regeneration from subcultured callus varied with the age and source of callus and differed from that reported in rice.
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Abbreviations
- MS:
-
Murashige and Skoog
- SEM:
-
scanning electron microscopy
- NAA:
-
1-naphthaleneacetic acid
- KIN:
-
kinetin
References
Chen CP, Lin CC, Namba T (1987) Shoyakugaku Zasshi 41: 215–225
Gyulai G, Janovszky J, Kiss E, Lelik L, Csillag A, Heszky LE (1992) Plant Cell Rep 11: 266–269
Han SH, Lee JS, Kwon OC (1990) The Research Reports Korea Agri Tech Res Inst 11: 13–19
Holdgate DP (1977) In: Reinert J, Bajaj YPS (eds) Plant Cell Tissue and Organ Culture, Springer-Verlag Berlin Heidelberg, pp 18–43
Ishioka N, Tanimoto S (1990) Plant Cell Tissue Organ Cult 22: 197–199
Kheterpal K, Khanna T, Arora RB (1989) J Ethnopharmacol 26: 183–187
Klimaszewska K, Keller WA (1985) Plant Cell Tissue Organ Cult 4: 183–197
Kwon OC, Chung CH, Sato T, Taniguchi T, Maeda E (1992) JpnJ Crop Sci 61: 469–475
Maeda E, Chen MH, Inoue M (1986) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 2, Crops I, Springer-Verlag Berlin Heidelberg, pp 105–122
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nakamura T, Maeda E (1989) Jpn J Crop Sci 58: 395–403
Predieri S, Malavasi FFF, (1989) Plant Cell Tissue Organ Cult 17: 133–142
Tanimoto S, Harada H (1989) Plant Cell Physiol 30: 541–547
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Communicated by A. Komamine
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Sato, T., Kwon, O.C., Miyake, H. et al. Regeneration of plantlets from petiole callus of wild viola (Viola patrinii DC.). Plant Cell Reports 14, 768–772 (1995). https://doi.org/10.1007/BF00232919
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DOI: https://doi.org/10.1007/BF00232919