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Comparison of the properties of the ATP-sensitive K+ channels of pancreatic β-cells of lean and obese (ob/ob) C57BL/6J mice

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Summary

Cultures of pancreatic islet cells from obese and lean mice of the C57BL/6J strain were established and their secretory response to glucose stimulation was measured. Insulin secretion (as % of total cellular insulin content) from the cells of the obese mouse cultures was significantly higher than from lean mouse cells. The properties of the glucose- and ATP-sensitive potassium channels present in these cultured β-cells were compared using the cell-attached and the inside-out configurations of the patchclamp technique. The channels of both types of mouse were indistinguishable in terms of conductance, ionic selectivity, kinetic behavior, voltage dependence or sensitivity to glucose, ATP and ADP. It is concluded that the depolarized state and the hypersecretory response of obese mouse β-cells are not related to an altered behavior of their ATP-sensitive potassium channels.

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Authors and Affiliations

  1. Department of Biochemistry, University of Ottawa, K1H 8M5, Ottawa, Ontario, Canada

    Linda Fournier & Nicole Bégin-Heick

  2. Cell Systems, Institute for Biological Sciences, National Research Council, K1A OR6, Ottawa, Ontario, Canada

    James F. Whitfield & Jean-Louis Schwartz

Authors
  1. Linda Fournier
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  2. Nicole Bégin-Heick
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  3. James F. Whitfield
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  4. Jean-Louis Schwartz
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Additional information

This research was supported through a grant from the Canadian Diabetes Association. LAF is a recipient of a studentship from the Medical Research Council of Canada. We wish to thank R. Jefferey who prepared the cultures and developed the experimental protocol for the immunofluorescence technique. We are very grateful to G.A.R. Mealing for his criticisms of the manuscript and for being a resource person for LAF in conducting the electrophysiological experiments.

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Fournier, L., Bégin-Heick, N., Whitfield, J.F. et al. Comparison of the properties of the ATP-sensitive K+ channels of pancreatic β-cells of lean and obese (ob/ob) C57BL/6J mice. J. Membarin Biol. 129, 267–276 (1992). https://doi.org/10.1007/BF00232908

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  • DOI: https://doi.org/10.1007/BF00232908

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