Abstract
A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.
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References
Benatti Junior, R. (1986) As aplicações do Rami. Casa da Agricultura 8, 12–16.
Calderon-Urrca, A. (1988) Transformation of Manihot esculenta (Cassava) using Agrobacterinm tumefaciens and expression of the introduced foreign genes in transformed cell lines. M.Sc. — Thesis, Vrije Universiteit Brussel.
De Almeida, E.R.P., Gosscle, V., Muller, C.G., Dockx, J., Reynaerts, A., Botterman, J., Krebbers, E. and Timko, M.P. (1989) Transgenic expression of two marker genes under the control of an Arabdopsis rbc S promoter: sequences encoding the Rubisco transit peptide increase expression levels. Mol. Gen. Genet. 218, 78–86.
Gamborg, O.L., Miller, R.A and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158.
Gander, E.S., Holmstroem, K. O., De Paiva, G.R., De Castro, L.A.B., Carneiro, M. and Grossi de Sá, M.F. (1991) Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut). Plant Mol. Biol. 16, 437–448.
Jefferson, R.A. (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5, 387–405.
Reiss, B., Sprengel, R. and Schaller, H. (1984) Protein fusions with the kanamycin resistance gene from transposom Tn5. EMBO J. 3, 3317–3322.
Ribeiro Filho, J. (1968) Cultura do rami. Viçosa, Universidade Rural do Estado de Minas Gerais, Escola Superior de Agricultura, 65p.
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning, a handbook, 2nd edition. Cold Spring Harbor Laboratory Press.
Squibb, RL. (1954) Ramie- a high-protein forage for tropical areas. J.Br.Grassl. Soc. 9, 313–323.
Willimot, S.G. (1954) Ramie fiber: Its cultivation and development. World crops 6, 405–408.
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Communicated by R. N. Beachy
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Dusi, D.M.A., Dubald, M., de Almeida, E.R.P. et al. Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation. Plant Cell Reports 12, 625–628 (1993). https://doi.org/10.1007/BF00232812
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DOI: https://doi.org/10.1007/BF00232812