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Plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification

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Summary

Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 45. 0 ±5.1% (based on the reduction of triphenyl tetrazolium chloride)following warming and unloading. For regrowth, cells were plated on semi-solid medium replenished with 40%(w/v) starch for 2d prior to reculture. Cell suspensions were reestablished and plants were regenerated from recovered cells. Twenty eight plants set seeds in the greenhouse.

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Abbreviations

PVS:

plant vitrification solution

P:

preculture

LN:

liquid nitrogen

TTC:

triphenyl tetrazolium chloride

2,4-D:

2,4-dichlorophenoxyacetic acid

DMSO:

dimethyl sulfoxide

EG:

ethylene glycol

BSA:

bovine serum albumin

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Authors and Affiliations

  1. Department of Biological Science and Biotechnology, Hangzhou University, 310028, Hangzhou, P.R.China

    Chun-Nong Huang, Jun-Hui Wang & Qin-Feng Yan

  2. China National Rice Research Institute, 310006, Hangzhou, P.R.China

    Qiu-Sheng Yan & Xue-Qin Zhang

Authors
  1. Chun-Nong Huang
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  2. Jun-Hui Wang
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  3. Qiu-Sheng Yan
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  4. Xue-Qin Zhang
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  5. Qin-Feng Yan
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Communicated by I.K. Vasil

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Huang, CN., Wang, JH., Yan, QS. et al. Plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification. Plant Cell Reports 14, 730–734 (1995). https://doi.org/10.1007/BF00232657

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  • DOI: https://doi.org/10.1007/BF00232657

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