Abstract
Rapid micropropagation of Trichopus zeylanicus Gaertn. subsp. travancoricus Burkil ex Narayanan, a rare ethnomedicinal herb endemic to the Western Ghats of southern India, was achieved by culturing shoot tips (0.3–0.5 cm) of 2-month-old axenic seedlings on Woody Plant Medium. Among the cytokinins tested, only BAP induced callus-free multiple shoot bud formation, with a maximum of 8.5±0.4 buds per explant being obtained with 2.0 mg.l−1 BAP after 8 weeks of culture. Shoot tips containing proliferated buds were divided and subcultured on medium containing 0.2 mg.l−1 BAP to produce 12.0±1.0 shoots per explant in 6 weeks. Excision of buds after culture initiation, with subculture of the debudded basal tissue in 2 successive passages yielded 20.0±1.0 and 13.5±0.5 buds per explant respectively. Each bud cultured in turn for 4 weeks on WPM with 1.0 mg.l−1 BAP formed 3.8±0.4 secondary buds which were repeatedly recultured to increase bud production. Altogether this method enabled an estimated harvest of 7848 buds from a single shoot tip in 28 months. Shoots (3–5 cm) developed from bud cultures were rooted in half-strength WPM medium with 0.5 mg.l−1 each of NAA and IBA, and 90–100% of the rooted plants were established in the field after hardening. Micropropagated plants were grown to maturity free of defects in growth, morphological, flowering and seed set characteristics.
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Abbreviations
- WPM:
-
Woody Plant Medium (Lloyd and `McCown 1980)
- BAP:
-
6-benzylaminopurine
- 2-ip:
-
2-iso-pentenyladenine
- Kinetin:
-
6-furfurylaminopurine
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-naphthaleneacetic acid
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Communicated by G.C. Phillips
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Krishnan, P.N., Sudha, C.G. & Seeni, S. Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn., a rare ethnomedicinal plant. Plant Cell Reports 14, 708–711 (1995). https://doi.org/10.1007/BF00232652
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DOI: https://doi.org/10.1007/BF00232652