Summary
Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2–3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33–35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment.
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Burnett, L., Yarrow, S. & Huang, B. Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera . Plant Cell Reports 11, 215–218 (1992). https://doi.org/10.1007/BF00232537
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DOI: https://doi.org/10.1007/BF00232537