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Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata [L.] RAF.)

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Abstract

Halved shoot bases of Allium tuberosum Rottl. ex Spreng. proliferated both axillary and adventitious shoots on B5 medium (1968) supplemented with either 6-benzylaminopurine (0.5 mg/l) or 1-naphthalene acetic acid (0.1 mg/l) and 2-isopentenyladenine (0.5 mg/l). In vitro shoots proliferated further numerous shoots upon subculture to fresh medium, and these shoots rooted spontaneously. Plantlets were transplanted successfully to soil and retained the diploid condition of the parents.

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Abbreviations

B5:

Gamborg et al. (1968) medium

BAP:

6-benzylaminopurine

2ip:

2isopentenyladenine

NAA:

1-naphthalene acetic acid

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Authors and Affiliations

  1. National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India

    J. Radhamani & K. P. S. Chandel

Authors
  1. J. Radhamani
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  2. K. P. S. Chandel
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Communicated by G. C. Phillips

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Radhamani, J., Chandel, K.P.S. Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata [L.] RAF.). Plant Cell Reports 11, 204–206 (1992). https://doi.org/10.1007/BF00232534

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  • DOI: https://doi.org/10.1007/BF00232534

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