Summary
Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (ß-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segments were subsequently tested in a leaf explant system. On leaves the transformation efficiency of the strains was much lower than with stems. Both the T-6b gene and the hsp 70-T-6b gene (a modified T-6b gene under the control of a heat shock promoter) allowed the initiation of GUS-positive buds.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- GUS:
-
ß-glucuronidase
- BAP:
-
benzylaminopurine
- X-gluc:
-
5-bromo-4-chloro-3-indolyl glucuronide
References
Baribault TJ, Skene KGM, Cain PA, Scott NS (1990) Exp Bot 229:1045–1049
Bonnard G, Tinland B, Paulus F, Szegedi E, Otten L (1990) Mol Gen Genet, 223:1–10
Clog E, Bass P, Walter B (1990) Plant Cell Reports 8:726–728
Guellec V, David C, Branchard M, Tempé J (1990) Plant Cell Tissue and Organ Culture, 20:211–215
Hemstad PR, Reisch BI (1985) J Plant Physiol 120:9–17.
Huss B, Tinland B, Paulus F, Walter B, Otten L (1990) Plant Mol Biol 14:173–186
Jefferson RA (1987) Plant Mol Biol Reptr 5:387–405
Kares C, Prinsen E, Van Onckelen H, Otten L (1990) Plant Mol Biol 15:225–236
Leemans J, Hernalsteens JP, Deblaere R, De Greve H, ThiaToong L, Van Montagu M, Schell J (1983) Genetics of Bacteria-Plant interaction. A. Pühler (ed.) Springer, Berlin
Martin C, Vernoy R, Carré M, Vesselle G, Collas A, Bougerey C (1987) Bull OIV 675–676:447–458
Mullins MG, Tang FCA, Facciotti D (1990) Biotechnology 8:1041–1046
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Panagopoulos CG, Psallidas PG (1973) J Appl Bacteriol 36:233–240
Rosenberg C, Boistard P, Dénarié J, Casse-Delbart F (1981) Mol Gen Genet 184:326–333
Tinland B (1990) Ph D Thesis University of Strasbourg
Tinland B, Huss B, Paulus F, Bonnard G, Otten L (1989) Mol Gen Genet 219:217–224
Tinland B, Rohfritsch O, Michler P, Otten (1990) Mol Gen Genet 223:1–10
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Berres, R., Otten, L., Tinland, B. et al. Transformation of vitis tissue by different strains of Agrobacterium tumefaciens containing the T-6b gene. Plant Cell Reports 11, 192–195 (1992). https://doi.org/10.1007/BF00232531
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232531