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Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose

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Summary

Anthers from spring wheat (Triticum aestivum L.) genotypes, including six F1 hybrids, were cultured in a modified liquid N6 medium containing either sucrose or maltose. In every case, use of maltose resulted in greater microspore callus induction and green shoot regeneration than culture in sucrose-containing medium. Induction in maltose medium also allowed green shoots to be recovered from crosses that showed only a poor response in other media and from two genotypes that did not respond to modified N6 medium with sucrose. Replacement of sucrose with maltose generally resulted in microspores having a more embryogenic mode of development in which distinct embryoids often formed. The most responsive genotype produced over 200 green shoots/100 anthers when cultured in medium with maltose.

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Author notes

  1. Pierre Hucl

    Present address: Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Agricultural Research and Development, Saskatchewan Wheat Pool, 207-112 Research Drive, P.O. Box 1461, S7K 3P7, Saskatoon, Saskatchewan, Canada

    Brian R. Orshinsky, Linda J. McGregor, Grace I. E. Johnson & Pierre Hucl

  2. Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada

    Kutty K. Kartha

Authors
  1. Brian R. Orshinsky
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  2. Linda J. McGregor
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  3. Grace I. E. Johnson
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  4. Pierre Hucl
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  5. Kutty K. Kartha
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Additional information

Communicated by F. Constabel

NRCC publication no. 31494

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Orshinsky, B.R., McGregor, L.J., Johnson, G.I.E. et al. Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose. Plant Cell Reports 9, 365–369 (1990). https://doi.org/10.1007/BF00232400

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  • DOI: https://doi.org/10.1007/BF00232400

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