Summary
Adaptation of protoplast regeneration systems for use on untested or recalcitrant potato genotypes can be a time-consuming exercise. Callus growth and xylogenesis were evaluated as early correlates of shooting potential to shorten this process. Callus growth was of limited value for predicting organogenesis but a linear relationship was observed between xylogenesis and shooting frequency. Increases in xylem content above a minimum threshold corresponded with increases in shooting frequency. The predictive value of the relationship was tested using a simple protocol modification (the culture of calli on a filter paper base). Calli on filter paper produced more xylem elements and shoots than those plated directly on medium. The potential of xylem content as a predictive test of shooting frequency is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bingham ET, Hurley LM, Kaatz DM, Saunders JW (1975) Crop Sci. 15: 719–721
Cassells AC (1979) Physiolog.P. 46: 159–164
Chen HR, Galston AW (1967) Physiolog. Pl. 20: 533–539
Coleman M, Davie P, Vessey J, Powell W (1991) Ann. Bot. 7: 459–461
Dai C, Mertz D, Lambeth V (1987) Plant Sci. 50: 79–84
Foulger D, Jones MGK (1986) Plant Cell Reports 5: 72–76
George EF, Sherrington PD (1984) Plant propagation by tissue culture. Handbook and directory of commercial laboratories. Eastern Press, Reading, p27
Mollers C, Zhang S, Wenzel G (1992) Plant Breeding 108: 12–18
Murashige T, Skoog F (1962) Physiolog. Pl. 15: 473–497
Payne LA, Barr SNR, Wilkinson MJ (1993) Proceedings of the Irish botanists meeting, University College, Cork. p.68
Seagull RW, Falconer MM (1991) In: Lloyd CW (ed) The cytoskeletal basis of plant growth and form, Academic Press, London, pp 183–194
Shahin EA (1984) In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1. Academic Press, London, pp 381–390
Shepard JF (1982) Plant Science Letters 26: 127–132
Shepard JF, Totten RE (1977) Pl. Physiol. 60: 313–316
Taylor RJ, Secor GA (1988) Potato Res. 31: 651–658
Taylor RJ, Secor GA (1990) Environmental and Experimental Botany 30: 355–359
Taylor RJ, Secor GA (1992) Environmental and Experimental Botany 32: 43–48
Wenzel G (1979) Proc. 5th Intl. Protoplast Symp. Hungarian Acad. Sciences, pp 327–339
Author information
Authors and Affiliations
Additional information
Communicated by M. R. Davey
Rights and permissions
About this article
Cite this article
Barr, S.N.R., Payne, L.A., Dale, M.F.B. et al. Predictive correlates of shoot regeneration from potato protoplast culture. Plant Cell Reports 15, 350–354 (1996). https://doi.org/10.1007/BF00232370
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232370