Abstract
Callus of three superior Eucalyptus grandis hybrids was induced from immature inflorescences, floral parts, shoot tips, zygotic embryos, and hypocotyl explants on various auxin (2,4-D or NAA) and cytokinin (kinetin) supplemented media. Hypocotyl callus initiated on 4 mg/l NAA and 1 mg/l kinetin formed massive nodular structures, and shoots and roots after four weeks on hormone free-medium. Callus from all other expiants turned brown and died upon transfer to hormone free or reduced hormone media. The nodular structures originating from hypocotyl-callus were maintained by subculture for over three years and retained the ability to form thousands of shoots. Shoots were successfully rooted (98% rooting) and plantlets developed were transferred to mist-greenhouse and then to greenhouse conditions with 95% survival. Plantlets were grown for six months in the greenhouse without sign of abnormal growth.
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Abbreviations
- NAA:
-
naphthalene acetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- MS:
-
Murashige and Skoog Medium (1962)
- IBA:
-
indolebutyric acid
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Warrag, E., Lesney, M.S. & Rockwood, D.J. Nodule culture and regeneration of Eucalyptus grandis hybrids. Plant Cell Reports 9, 586–589 (1991). https://doi.org/10.1007/BF00232338
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DOI: https://doi.org/10.1007/BF00232338