Summary
Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10−6M kinetin + 10−5M α -naphthalene acetic acid. Suspension cultures were initiated from the calli in a similar medium but with 10−5M 2,4-dichlorophenoxy acetic acid in place of α -naphthalene acetic acid. In ca 80% of the suspension cultures somatic embryos differentiated freely (80–85%) as well as on the surface of small clumps of tissue (15–20%). Somatic embryos that developed beyond heart-shaped stage were transferred to agar-solidified Murashige & Skoog's medium free of growth substances. When maintained in 10 h light and 14 h dark the somatic embryos developed into plantlets bearing cauline leaves. From seed sowing to raising normal plantlets via callus required 28 weeks; on average 80 plantlets were obtained from one explant in three passages.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- FAA:
-
formalin-acetic acid-alcohol
- GA3 :
-
gibberellic acid
- Kn:
-
kinetin
- MS:
-
Murashige & Skoog's medium (Murashige and Skoog 1962)
- NAA:
-
α -naphthalene acetic acid
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Communicated by R.N. Beachy
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Sulaiman, I.M., Rangaswamy, N.S. & Babu, C.R. Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae). Plant Cell Reports 9, 582–585 (1991). https://doi.org/10.1007/BF00232337
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DOI: https://doi.org/10.1007/BF00232337