Abstract
Cotyledons of various ages from seedlings of eight watermelon (Citrullus vulgaris) cultivars were cultured on MS medium supplemented with different combinations of phytohormones. High frequency shoot regeneration (60.0–92.0%) was induced from 5-day-old cotyledons of cultivars cultured on MS medium containing 5.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l indole-3-acetic acid (IAA). Multiple shoot buds elongated on MS medium containing 0.2 mg/l kinetin (KT) and 5–10 shoots per expiant could be recovered depending on the cultivars. Elongated shoots rooted on MS medium with 0.1 mg/l α-naphthalene acetic acid (NAA). Zeatin riboside (ZT) had a similar efficiency as BA in shoot induction, and it was significantly more functional than 2-isopentenyladenine (2iP) or kinetin (KT). Cotyledons from 5-day-old seedlings were the most responsive to shoot induction.
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Abbreviations
- BA:
-
6-benzylaminopurine
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- 2iP:
-
2-isopentenyladenine
- KT:
-
kinetin
- MS:
-
Murashige and Skoog (1962)
- NAA:
-
α-naphthalene acetic acid
- ZT:
-
zeatin riboside
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Communicated by I.K. Vasil
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Dong, JZ., Jia, SR. High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad). Plant Cell Reports 9, 559–562 (1991). https://doi.org/10.1007/BF00232331
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DOI: https://doi.org/10.1007/BF00232331