Summary
A system has been developed for efficient regeneration of shoots from Brassica campestris in vitro. Using 4-day old cotyledons with petioles as expiants and a combination of BA and NAA in the regeneration media, up to 70% of expiants produced shoots after 2 weeks in culture. The optimal conditions for regeneration were found to include a BA concentration of 2mgL−1 and NAA concentration of 1mgL−1. Light intensity had a profound effect on regeneration potential. The use of silver ions as an inhibitor of ethylene action reduced regeneration rates in this system. Rooting occured simultaneously with shoot formation on these media and the resultant shoots could be rooted readily on minimal medium. The genotype dependency was investigated and indicated that this method would be widely applicable to B. campestris cultivars. Regeneration of one cultivar, a high erucic acid type (R-500), was inefficient in the system described here. Histological studies indicated the development of multiple shoot primordia from the petiolar cut ends of the expiants after the initiation of meristematic activity in the cells about 100μm from the cut site within 2 days of culture initiation. The system described is compatible with previously reported Agrobacterium — mediated transformation protocols involving cotyledonary petioles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chi, G-L and E-C Pua (1989). Plant Science 64: 243–450.
De Block, M, D. De Brouwer, and P. Tenning (1989). Plant Physiology 91: 694–701.
Dietert, M.F., S.A. Barron and O.C. Yoder (1982). Plant Science Letters 26: 233–240.
Dunwell, J.M. (1981). Journal of Experimental Botany 32: 789–799.
Glimelius, K. (1984). Physiolgia Plantarum 61: 38–44.
Jain, R.K., J.B. Chowdhury, D.R. Sharma and W. Friedt (1988). Plant Cell, Tissue and Organ Culture 14: 197–206.
Jaiswal, S.K., S. S. Bhojwani and S.P. Bhatnager (1987). Phytomorphology 37: 235–241.
Keller, W.A. and K.C. Armstrong (1979). Theoretical and Applied Genetics 55: 65–67.
Lennox, G. (1984). in An Overview of the Canadian Oilseed Industry. Statistics Canada. ISBN 0 — 660 — 11658 — 8E.
Monteiro, A.A. and W.H. Gabelman (1988). Horticultural Science 23: 876–877.
Moloney, M.M, J.M. Walker and K.K. Sharma (1989). Plant Cell Reports 8: 238–242.
Murashige, T. and F. Skoog (1962). Physiolgia Plantarum 15: 493–497.
Murata, M. and T.J. Orton (1987). Plant Cell, Tissue and Organ Culture 11: 111–123.
Narasimhulu, S.B. and V.L. Chopra (1988). Plant Cell Reports 7: 104–106.
O'Brien, T.P. and M.E. McCully (1981). The Study of Plant Structure: Principles and Selected Methods. Termarcarphi Pty. Ltd., Melbourne.
Sharma, K.K. and S.S. Bhojwani (1990). Plant Science 69: 207–214.
Sharma, K.K., S.S. Bhojwani and T.A. Thorpe (1990a). Plant Science 66: 247–253.
Sharma, K.K., S.S. Bhojwani and T.A. Thorpe (1990b). Plant Cell, Tissue and Organ Culture (in press).
Skoog, F. and C.O. Miller (1957). Symposia of the Society for Experimental Biology 11: 118–131.
Yeung, E.C. (1984). in Cell Culture and Somatic Cell Genetics of Plants. ed. Indra K. Vasil. Volume 1. Laboratory Procedures and Their Applications. Acedemic Press. pp. 689–697.
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Hachey, J.E., Sharma, K.K. & Moloney, M.M. Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Reports 9, 549–554 (1991). https://doi.org/10.1007/BF00232329
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232329