Skip to main content
SpringerLink
Log in

A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this height, appeared highly correlated with the fresh weight of cells. This result was found with eight cell lines in two Laboratories. The method proved to be very suitable to allow routinely measurement of FW without the destruction of cells, from many samples, in a short time, during each phase of the growth cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CVS:

cell volume after sedimentation

FW:

fresh weight of cells

References

  • Gamborg OL, Miller RA, Ojima M. (1968) Exp. Cell Res. 50: 151–158

    Google Scholar 

  • Gilissen LJW, Hänisch ten Cate CH, Keen, B (1983) Plant Cell Rep. 2: 232–235

    Google Scholar 

  • Kreis W, Reinhard E, (1988) Planta Med. 54: 95–100

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127

    Google Scholar 

  • Mérillon JM, Ouelhazi L, Doireau P, Chénieux JC, Rideau M (1989) J. Plant Physiol. 134: 54–60

    Google Scholar 

  • Morris P, Rudge K, Cresswell R, Fowler MW (1989) Plant Cell Tissue Organ Cult. 17: 79–90

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant 15: 473–479

    Google Scholar 

  • Schripsema J, Meyer AH, van Iren F, ten Hoopen HJG, Verpoorte R (1990) Plant Cell Tissue Organ Cult. 22: 55–64

    Google Scholar 

  • Scragg AH, Allan EJ, Morris P (1988) J. Plant Physiol. 132: 184–189

    Google Scholar 

  • Street HE, (1977) In: Street HE. (ed) Botanical monographs, Vol 11, Blackwell Scientific Publications, Oxford, pp 61–102

    Google Scholar 

  • Wilson, G, Balagué, C (1985) J. Exp. Bot. 36: 485–493

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands

    T. J. M. Blom, F. van Iren & K. R. Libbenga

  2. Pharmazeutisches Institut, Eberhard-Karls-Universität, Auf der Morgenstelle 8, W-7400, Tübingen, FRG

    W. Kreis

Authors
  1. T. J. M. Blom
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Kreis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. van Iren
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. R. Libbenga
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by N. Amrhein

Rights and permissions

Reprints and permissions

About this article

Cite this article

Blom, T.J.M., Kreis, W., van Iren, F. et al. A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures. Plant Cell Reports 11, 146–149 (1992). https://doi.org/10.1007/BF00232168

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232168

Keywords

Search

Navigation